AC   MF_00970;
DC   Protein; auto
TR   HAMAP; MF_00970; -; 1; level=0
XX
Names: RNase_E
XX
ID   RNE
DE   RecName: Full=Ribonuclease E;
DE            Short=RNase E;
DE            EC=3.1.26.12;
GN   Name=rne;
XX
CC   -!- FUNCTION: Endoribonuclease that plays a central role in RNA processing
CC       and decay. Required for the maturation of 5S and 16S rRNAs and the
CC       majority of tRNAs. Also involved in the degradation of most mRNAs.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of single-stranded RNA in A- and U-
CC         rich regions.; EC=3.1.26.12;
case <FTGroup:2>
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC       Note=Binds 2 Zn(2+) ions per homotetramer.;
end case
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Note=Binds 1 Mg(2+) ion per subunit.;
end case
case <OC:Gammaproteobacteria>
CC   -!- SUBUNIT: Component of the RNA degradosome, which is a multiprotein
CC       complex involved in RNA processing and mRNA degradation. Within the RNA
CC       degradosome, RNase E assembles into a homotetramer formed by a dimer of
CC       dimers.
else
CC   -!- SUBUNIT: Homotetramer formed by a dimer of dimers.
end case
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Cell inner membrane; Peripheral
CC       membrane protein; Cytoplasmic side.
CC   -!- SIMILARITY: Belongs to the RNase E/G family. RNase E subfamily.
XX
DR   PROSITE; PS50126; S1; 1; trigger=yes
DR   Pfam; PF12111; PNPase_C; 0-1; trigger=no
DR   Pfam; PF10150; RNase_E_G; 1; trigger=no
DR   Pfam; PF00575; S1; 1; trigger=no
DR   NCBIfam; TIGR00757; RNaseEG; 1; trigger=no
XX
KW   Cell inner membrane
KW   Cell membrane
KW   Cytoplasm
KW   Endonuclease
KW   Hydrolase
case <FTGroup:1>
KW   Magnesium
end case
KW   Membrane
case <FTGroup:1> or <FTGroup:2>
KW   Metal-binding
end case
KW   Nuclease
KW   RNA-binding
KW   rRNA processing
KW   tRNA processing
KW   rRNA-binding
KW   tRNA-binding
case <FTGroup:2>
KW   Zinc
end case
XX
GO   GO:0004521; F:RNA endonuclease activity
case <FTGroup:1>
GO   GO:0000287; F:magnesium ion binding
end case
GO   GO:0003723; F:RNA binding
case <FTGroup:2>
GO   GO:0008270; F:zinc ion binding
end case
GO   GO:0006402; P:mRNA catabolic process
GO   GO:0006364; P:rRNA processing
GO   GO:0008033; P:tRNA processing
GO   GO:0005737; C:cytoplasm
GO   GO:0009898; C:cytoplasmic side of plasma membrane
XX
FT   From: RNE_ECOLI (P21513)
FT   REGION          404..407
FT                   /note="Required for zinc-mediated homotetramerization and
FT                   catalytic activity"
FT   Condition: C-x-x-C
FT   BINDING         303
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT   Group: 1; Condition: D
FT   BINDING         346
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT   Group: 1; Condition: D
FT   BINDING         404
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="ligand shared between dimeric partners"
FT   Group: 2; Condition: C
FT   BINDING         407
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="ligand shared between dimeric partners"
FT   Group: 2; Condition: C
XX
Size: 560-1230;
Related: None;
Template: P21513;
Scope:
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.12 2023/06/01
//