AC   MF_00993;
DC   Protein; auto
TR   HAMAP; MF_00993; -; 1; level=0
XX
Names: MqnE
XX
ID   MQNE
DE   RecName: Full=Aminodeoxyfutalosine synthase;
DE            Short=AFL synthase;
DE            Short=Aminofutalosine synthase;
DE            EC=2.5.1.120;
DE   AltName: Full=Menaquinone biosynthetic enzyme MqnE;
GN   Name=mqnE;
XX
CC   -!- FUNCTION: Radical SAM enzyme that catalyzes the addition of the
CC       adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate,
CC       leading to aminodeoxyfutalosine (AFL), a key intermediate in the
CC       formation of menaquinone (MK, vitamin K2) from chorismate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-[(1-carboxyvinyl)-oxy]benzoate + H2O + S-adenosyl-L-
CC         methionine = 6-amino-6-deoxyfutalosine + H(+) + hydrogencarbonate +
CC         L-methionine; Xref=Rhea:RHEA:33075, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:57844,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:64286, ChEBI:CHEBI:76981;
CC         EC=2.5.1.120;
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.;
end case
CC   -!- PATHWAY: Quinol/quinone metabolism; menaquinone biosynthesis.
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. MqnE family.
XX
DR   Pfam; PF04055; Radical_SAM; 1; trigger=no
DR   NCBIfam; TIGR03700; mena_SCO4494; 1; trigger=no
DR   NCBIfam; TIGR00423; TIGR00423; 1; trigger=no
DR   PIRSF; PIRSF004762; CHP00423; 1; trigger=no
DR   PROSITE; PS51918; RADICAL_SAM; 1; trigger=yes
XX
case <FTGroup:1>
KW   4Fe-4S
KW   Iron
KW   Iron-sulfur
KW   Metal-binding
KW   S-adenosyl-L-methionine
end case
KW   Menaquinone biosynthesis
KW   Transferase
XX
case <FTGroup:1>
GO   GO:0005506; F:iron ion binding
end case
GO   GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups
GO   GO:0009234; P:menaquinone biosynthetic process
XX
FT   From: MQNE_THET8 (Q5SK48)
FT   BINDING         69
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 1; Condition: C
FT   BINDING         73
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 1; Condition: C
FT   BINDING         76
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT   Group: 1; Condition: C
XX
Size: 335-420;
Related: None;
Template: Q5SK48;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.13 2023/06/01
//