AC   MF_01885;
DC   Protein; auto
TR   HAMAP; MF_01885; -; 1; level=0
XX
Names: tRNA_methyltr_TrmL
XX
ID   TRML
case <OC:Pseudomonadota>
DE   RecName: Full=tRNA (cytidine(34)-2'-O)-methyltransferase;
DE            EC=2.1.1.207;
DE   AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL;
GN   Name=trmL;
else
DE   RecName: Full=Putative tRNA (cytidine(34)-2'-O)-methyltransferase;
DE            EC=2.1.1.207;
DE   AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase;
end case
XX
case <OC:Pseudomonadota>
CC   -!- FUNCTION: Methylates the ribose at the nucleotide 34 wobble position in
CC       the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA).
CC       Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH
CC       of the wobble nucleotide.
else
CC   -!- FUNCTION: Could methylate the ribose at the nucleotide 34 wobble
CC       position in tRNA.
end case
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cytidine(34) in tRNA + S-adenosyl-L-methionine = 2'-O-
CC         methylcytidine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43084, Rhea:RHEA-COMP:10331, Rhea:RHEA-COMP:10332,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74495, ChEBI:CHEBI:82748; EC=2.1.1.207;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S-
CC         adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-
CC         methyluridine(34) in tRNA(Leu) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43088, Rhea:RHEA-COMP:10333, Rhea:RHEA-COMP:10334,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74508, ChEBI:CHEBI:74511; EC=2.1.1.207;
case <OC:Pseudomonadota>
CC   -!- SUBUNIT: Homodimer.
end case
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. RNA methyltransferase TrmH family. TrmL subfamily.
XX
DR   Pfam; PF00588; SpoU_methylase; 1; trigger=no
DR   NCBIfam; TIGR00185; RRNA_methyl_2; 1; trigger=no
DR   PIRSF; PIRSF029256; SpoU_TrmH_prd; 1; trigger=no
XX
KW   Cytoplasm
KW   Methyltransferase
KW   S-adenosyl-L-methionine
KW   Transferase
KW   tRNA processing
XX
GO   GO:0008175; F:tRNA methyltransferase activity
GO   GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity
GO   GO:0030488; P:tRNA methylation
GO   GO:0005737; C:cytoplasm
XX
FT   From: TRML_HAEIN (P44868)
FT   BINDING         78
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: [ILVM]
FT   BINDING         100
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: G
FT   BINDING         122
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: [ILVM]
FT   BINDING         130
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: S
XX
Size: 140-230;
Related: None;
Template: P0AGJ7; P44868; Q74Y93;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: in AZOS1
Comments: None
XX
# Revision 1.12 2023/06/01
//