AC   MF_00059;
DC   Protein; auto
TR   HAMAP; MF_00059; -; 1; level=0
XX
Names: RNApol_bact_RpoA
XX
ID   RPOA
case not <OG:Chloroplast>
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha;
DE            Short=RNAP subunit alpha;
DE            EC=2.7.7.6;
DE   AltName: Full=RNA polymerase subunit alpha;
DE   AltName: Full=Transcriptase subunit alpha;
end case
case <OG:Chloroplast>
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha;
DE            Short=PEP;
DE            EC=2.7.7.6;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit alpha;
DE            Short=RNA polymerase subunit alpha;
end case
GN   Name=rpoA;
XX
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
case not <OG:Chloroplast> and not <OC:Cyanobacteriota>
CC   -!- SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1
CC       beta, 1 beta' and 1 omega subunit. When a sigma factor is associated
CC       with the core the holoenzyme is formed, which can initiate
CC       transcription.
end case
case <OC:Cyanobacteriota>
CC   -!- SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2
CC       alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma
CC       factor is associated with the core the holoenzyme is formed, which can
CC       initiate transcription.
end case
case <OG:Chloroplast>
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
end case
CC   -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal
CC       transcription, whereas the C-terminal domain is involved in interaction
CC       with transcriptional regulators and with upstream promoter elements.
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
XX
DR   Pfam; PF01000; RNA_pol_A_bac; 1; trigger=no
DR   Pfam; PF03118; RNA_pol_A_CTD; 1; trigger=no
DR   NCBIfam; TIGR02027; rpoA; 1; trigger=no
XX
KW   Transferase
KW   Nucleotidyltransferase
KW   Transcription
KW   DNA-directed RNA polymerase
XX
GO   GO:0003677; F:DNA binding
GO   GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity
GO   GO:0006351; P:DNA-templated transcription
case <OG:Chloroplast>
GO   GO:0009507; C:chloroplast
end case
XX
FT   From: RPOA_ECOLI (P0A7Z4)
FT   REGION          Nter..235
FT                   /note="Alpha N-terminal domain (alpha-NTD)"
FT   REGION          249..Cter
FT                   /note="Alpha C-terminal domain (alpha-CTD)"
XX
Size: 309-383;
Related: None;
Template: P0A7Z4; Q925Z2; Q9KWU8; Q9Z9H6;
Scope:
 Bacteria
 Plastid
Fusion:
 Nter: None
 Cter: None
Duplicate: in FRATF, FRATH, FRATO, FRATN, FRATT, FRAT1, FRATW, LEPBL, PSYIN,
 STRAW
Plasmid: None
Comments: Divergent BIGNA, CARRP, CHLRE, CHLVU, HELSJ, NEPOL, ONYPE,
 OLTVI, PELHO, PSEAK, SCEOB, STIHE and Euglids not used in alignment;
 they are either quite short or have inserts. In PELHO there are 3
 rpoA-like genes, but it is not clear if any of them are functional.
XX
# Revision 1.48 2023/06/01
//