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HAMAP annotation rule: MF_00100
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General rule information

Accession MF_00100
Dates 1-JUN-2001 (Created)
16-MAY-2011 (Last updated, Version 44)
Data class Protein

case <OC:Bacteria> or <OG:Chloroplast>
Predictors HAMAP; MF_00100_B; [distribution of match scores in UniProtKB];[seed alignment for MF_00100_B]
end case


case <OC:Archaea>
Predictors HAMAP; MF_00100_A; [distribution of match scores in UniProtKB];[seed alignment for MF_00100_A]
end case

Names IF-2


Propagated annotation

Identifier, protein and gene names

case <OC:Bacteria>
Identifier IF2
Protein name
RecName: Full=Translation initiation factor IF-2;
end case


case <OG:Chloroplast>
Identifier IF2C
Protein name
RecName: Full=Translation initiation factor IF-2, chloroplastic;
end case


case <OC:Archaea>
Identifier IF2P
Protein name
RecName: Full=Probable translation initiation factor IF-2;
end case

Gene name infB
Comments

case <OC:Bacteria>
FUNCTION: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
end case


case <OC:Archaea>
FUNCTION: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).
end case


case <OG:Chloroplast>
FUNCTION: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity).
SUBCELLULAR LOCATION: Plastid, chloroplast.
end case

SIMILARITY: Belongs to the IF-2 family.
Cross-references
Pfam PF00009; GTP_EFTU; 1;
TIGRFAMs TIGR00487; IF-2; 1;
TIGR00491; AIF-2; 1;
PROSITE PS01176; IF2; 1;
Keywords

case <FT:5>
Acetylation.
end case


case <OC:Bacteria>
Cytoplasm.
end case

Initiation factor, Protein biosynthesis, GTP-binding, Nucleotide-binding.
Gene Ontology
GO:0003924; Molecular function: GTPase activity.
GO:0003743; Molecular function: translation initiation factor activity.
GO:0006413; Biological process: translational initiation.

case <OC:Bacteria>
GO:0005737; Cellular component: cytoplasm.
end case


case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Features

case <OC:Bacteria> or <OG:Chloroplast>
From: IF2_ECOLI (P0A705)
Key     From     To       Description   Condition   FTGroup
NP_BIND     398     405       GTP (By similarity)   G-H-V-D-H-G-K-T  
NP_BIND     444     448       GTP (By similarity)   D-T-P-G-H  
NP_BIND     498     501       GTP (By similarity)   [NST]-K-[LIVCMT]-D  
REGION     392     540       G-domain      
end case

case <OC:Escherichia> or <OC:Shigella>
Key     From     To       Description   Condition   FTGroup
MOD_RES     808     808       N6-acetyllysine (By similarity)   K  
end case

case <OC:Archaea>
From: IF2P_METTH (O26359)
Key     From     To       Description   Condition   FTGroup
NP_BIND     12     19       GTP (By similarity)   G-H-V-D-[HVA]-G-K-T  
NP_BIND     76     80       GTP (By similarity)   D-T-P-G-H  
NP_BIND     130     133       GTP (By similarity)   [NT]-K-[LIV]-D  
end case



Additional information


case <OC:Bacteria>
Size range: 571-1183 amino acids
end case


case <OC:Archaea>
Size range: 589-617 amino acids
end case

Related UniRules: None
Template: P04766 (IF2_GEOSE); P0A705 (IF2_ECOLI); P48515 (IF2_THET8); Q9HNQ2 (IF2P_HALSA); O26359 (IF2P_METTH): [Recover all]
Scope: Bacteria
Archaea
Plastid
Fusion: Nter: None; Cter: None
Duplicate: None
Plasmid encoded: None
Comments: Highly divergent N-terminal sequences. Inteins deleted in METJA, PYRAB, PYRFU and PYRHO.

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UniProtKB rule member sequences