HAMAP

Accession	MF_00554
Dates	9-MAY-2002 (Created) 27-JAN-2011 (Last updated, Version 17)

Data class

Protein

Predictors

HAMAP; MF_00554; [distribution of match scores in UniProtKB];[seed alignment for MF_00554]

Names

NEP1

Identifier

NEP1

Protein name

RecName: Full=Ribosomal RNA small subunit methyltransferase Nep1; EC=2.1.1.-; AltName: Full=16S rRNA (pseudouridine-N1-)-methyltransferase Nep1;

Gene name

nep1

FUNCTION: Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA (By similarity).

CATALYTIC ACTIVITY: S-adenosyl-L-methionine + a pseudouridine in 16S rRNA = S-adenosyl-L-homocysteine + a N(1)-methylpseudouridine in 16S rRNA.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the NEP1 family.

Pfam	PF03587; EMG1; 1;

Methyltransferase, Ribosome biogenesis, RNA-binding, rRNA-binding, rRNA processing, S-adenosyl-L-methionine, Transferase.

GO:0019843; Molecular function: rRNA binding.
GO:0070037; Molecular function: rRNA (pseudouridine) methyltransferase activity.
GO:0042254; Biological process: ribosome biogenesis.
GO:0006364; Biological process: rRNA processing.

From: NEP1_METJA (Q57977)
Key		From		To		Description		Condition		FTGroup
REGION		185		190		S-adenosyl-L-methionine binding (By similarity)
BINDING		162		162		S-adenosyl-L-methionine; via amide nitrogen (By similarity)		G
BINDING		167		167		S-adenosyl-L-methionine; via carbonyl oxygen (By similarity)		G
SITE		54		54		Interaction with substrate rRNA (By similarity)		R
SITE		56		56		Stabilizes Arg-xx (By similarity)		D
SITE		95		95		Interaction with substrate rRNA (By similarity)		R
SITE		98		98		Interaction with substrate rRNA (By similarity)		[RK]
SITE		102		102		Interaction with substrate rRNA (By similarity)		[RN]

Size range:	205-232 amino acids
Related UniRules:	None
Template:	Q57977 (NEP1_METJA); Q06287 (NEP1_YEAST): [Recover all]
Scope:	Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None