AC   MF_01057;
DC   Protein; auto
TR   HAMAP; MF_01057; -; 1; level=0
XX
Names: tRNA_methyltr_TrmB
XX
ID   TRMB
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase;
DE            EC=2.1.1.33;
DE   AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase;
DE   AltName: Full=tRNA(m7G46)-methyltransferase;
GN   Name=trmB;
XX
CC   -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at position 46
CC       (m7G46) in tRNA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-
CC         methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189,
CC         ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269,
CC         ChEBI:CHEBI:74480; EC=2.1.1.33;
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
case <OC:Enterobacterales>
CC   -!- SUBUNIT: Monomer.
end case
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. TrmB family.
XX
DR   PROSITE; PS51625; SAM_MT_TRMB; 1; trigger=no
DR   Pfam; PF02390; Methyltransf_4; 1; trigger=no
DR   NCBIfam; TIGR00091; TIGR00091; 1; trigger=no
XX
KW   Methyltransferase
KW   S-adenosyl-L-methionine
KW   Transferase
KW   tRNA processing
XX
GO   GO:0008176; F:tRNA (guanine(46)-N7)-methyltransferase activity
GO   GO:0006400; P:tRNA modification
XX
FT   From: TRMB_ECOLI (P0A8I5)
FT   REGION          150..155
FT                   /note="Interaction with RNA"
FT   Optional; Condition: [KR]-[KH]-[EN]-K-R-R
FT   BINDING         217..220
FT                   /ligand="substrate"
FT   Optional; Condition: T-x-[YF]-E
FT   BINDING         69
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: [DE]
FT   BINDING         94
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: [DE]
FT   BINDING         121
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: [DEN]
FT   BINDING         144
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Optional; Condition: [DN]
FT   BINDING         148
FT                   /ligand="substrate"
FT   Optional; Condition: K
FT   BINDING         180
FT                   /ligand="substrate"
FT   Optional; Condition: D
XX
Size: 185-400;
Related: None;
Template: P0A8I5; O34522; P67506; O66479;
Scope:
 Bacteria
Fusion:
 Nter: hemK; kdtA
 Cter: None
Duplicate: None
Plasmid: in RUEPO
Comments: Fused to hemK in RICBR, RICCN, RICFE, RICPR and RICTY, and fused to
 kdtA in FUSNN; Unknown N-terminal domain in METPP; sequences not included in
 alignment and not taken into account in size range
XX
# Revision 1.31 2023/10/13
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