AC MF_01161; DC Protein; auto TR HAMAP; MF_01161; -; 1; level=0 XX Names: tRNA_Ile_lys_synt XX ID TILS case not DE RecName: Full=tRNA(Ile)-lysidine synthase; DE EC=6.3.4.19; DE AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase; DE AltName: Full=tRNA(Ile)-lysidine synthetase; else case DE RecName: Full=tRNA(Ile)-lysidine synthase, chloroplastic; DE EC=6.3.4.19; DE AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase; DE AltName: Full=tRNA(Ile)-lysidine synthetase; end case GN Name=tilS; XX CC -!- FUNCTION: Ligates lysine onto the cytidine present at position 34 of CC the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an CC ATP-dependent manner. Cytidine is converted to lysidine, thus changing CC the amino acid specificity of the tRNA from methionine to isoleucine. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + CC diphosphate + H(+) + lysidine(34) in tRNA(Ile2); CC Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, CC ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665, CC ChEBI:CHEBI:456215; EC=6.3.4.19; case CC -!- SUBCELLULAR LOCATION: Cytoplasm. else case CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast. end case case CC -!- DOMAIN: The N-terminal region contains the highly conserved SGGXDS CC motif, predicted to be a P-loop motif involved in ATP binding. end case CC -!- SIMILARITY: Belongs to the tRNA(Ile)-lysidine synthase family. XX DR Pfam; PF01171; ATP_bind_3; 1; trigger=no DR NCBIfam; TIGR02433; lysidine_TilS_C; 1; trigger=no DR NCBIfam; TIGR02432; lysidine_TilS_N; 1; trigger=no XX case KW ATP-binding KW Nucleotide-binding end case case KW Cytoplasm end case KW Ligase KW tRNA processing XX case GO GO:0005524; F:ATP binding end case GO GO:0016879; F:ligase activity, forming carbon-nitrogen bonds GO GO:0006400; P:tRNA modification case GO GO:0005737; C:cytoplasm else case GO GO:0009507; C:chloroplast end case XX FT From: TILS_ECOLI (P52097) FT BINDING 20..25 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: S-G-G-x-D-S XX Size: 278-648; Related: None; Template: P52097; Scope: Bacteria Plastid Fusion: Nter: None Cter: ; Duplicate: None Plasmid: None Comments: None XX # Revision 1.30 2023/06/01 //