HAMAP

Accession	MF_01259
Dates	21-APR-2005 (Created) 24-SEP-2010 (Last updated, Version 11)

Data class

Protein

Predictors

HAMAP; MF_01259; [distribution of match scores in UniProtKB];[seed alignment for MF_01259]

Names

F420_ligase_CofE_2

Identifier

COFE

Protein name

RecName: Full=F420-0:gamma-glutamyl ligase; EC=6.3.2.-;

Gene name

cofE

FUNCTION: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives (By similarity).

CATALYTIC ACTIVITY: GTP + F420-0 + n L-glutamate = GDP + phosphate + F420-n.

COFACTOR: Divalent cation. The ion could be magnesium (By similarity).

COFACTOR: Monovalent cation. The ion could be potassium (By similarity).

PATHWAY: Cofactor biosynthesis; coenzyme F420 biosynthesis.

SIMILARITY: In the N-terminal section; belongs to the CofE family.

Pfam	PF01996; F420_ligase; 1; PF00881; Nitroreductase; 1;
TIGRFAMs	TIGR01916; F420_puta_dedox; 1;

GTP-binding, Ligase, Manganese, Nucleotide-binding, Potassium.

GO:0016881; Molecular function: acid-amino acid ligase activity.
GO:0009108; Biological process: coenzyme biosynthetic process.

From: COFE_MYCBO (Q7TWV3)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		244		F420-0:gamma-glutamyl ligase
REGION		245		Cter		Unknown

Size range:	423-457 amino acids
Related UniRules:	MF_01258 (COFE)
Template:	Q7TWV3 (COFE_MYCBO)
Scope:	Bacteria; Mycobacterium Bacteria; Nocardia Bacteria; Streptomyces
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	CofE homologs are found in the genomes of all organisms currently known to produce coenzyme F420. These include not only the methanogenic archaea but also nonmethanogenic archaea such as Halobacteria and Archaeoglobus (see MF_01258), as well as some eubacteria such as Streptomyces, Mycobacterium, Nocardia, and Thermobifida.