HAMAP annotation rule MF_01259
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Accession MF_01259
Dates 21-APR-2005 (Created)
11-JUN-2012 (Last updated, Version 13)
Name F420_ligase_FbiB
Scope Bacteria; Mycobacterium
Bacteria; Nocardia
Bacteria; Streptomyces
Triggered by



Identifier
FBIB
Protein name
RecName: Full=Coenzyme F420:L-glutamate ligase;
EC=6.3.2.31;
EC=6.3.2.34;
AltName: Full=Coenzyme F420-0:L-glutamate ligase;
AltName: Full=Coenzyme F420-1:gamma-L-glutamate ligase;
Gene name
fbiB


case <OC:Mycobacterium>
FUNCTION Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives (By similarity).
else
FUNCTION Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives (By similarity).
end case
CATALYTIC ACTIVITY GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.
case <OC:Mycobacterium>
CATALYTIC ACTIVITY GTP + coenzyme F420-0 + n L-glutamate = GDP + phosphate + coenzyme gamma-F420-n.
end case
COFACTOR Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese (By similarity).
Monovalent cation. The ion could be potassium (By similarity).
PATHWAY Cofactor biosynthesis; coenzyme F420 biosynthesis.
SIMILARITY In the N-terminal section; belongs to the CofE family.


GTP-binding
Ligase
Magnesium
Manganese
Metal-binding
Nucleotide-binding
Potassium


GO:0052618; Molecular function: coenzyme F420-0:L-glutamate ligase activity.
GO:0052619; Molecular function: coenzyme F420-1:gamma-L-glutamate ligase activity.
GO:0009108; Biological process: coenzyme biosynthetic process.
GO:0052645; Biological process: F420-0 metabolic process.


Pfam PF01996; F420_ligase; 1;
PF00881; Nitroreductase; 1;
TIGRFAMs TIGR01916; F420_cofE; 1;
TIGR03553; F420_FbiB_CTERM; 1;


From: FBIB_MYCBO (Q7TWV3)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     244       F420-0:gamma-glutamyl ligase        
REGION     245     Cter       Unknown        
NP_BIND     20     23       GTP (By similarity)     [LIMV]-P-x-[IVEF]  
BINDING     50     50       GTP (By similarity)     S  
METAL     109     109       Divalent metal cation 1 (By similarity)     D  
METAL     150     150       Divalent metal cation 1 (By similarity)     D  
METAL     151     151       Divalent metal cation 2 (By similarity)     [TS]  
BINDING     55     55       GTP (By similarity)     K  
BINDING     112     112       GTP (By similarity)     N  


Size range: 423-457 amino acids
Related Rules: MF_01258 (COFE)
Templates: Q7TWV3 (FBIB_MYCBO); A0QTG1 (FBIB_MYCS2): [Recover all]
Fusion: None
Comments: CofE homologs are found in the genomes of all organisms currently known to produce coenzyme F420. These include not only the methanogenic archaea but also nonmethanogenic archaea such as Halobacteria and Archaeoglobus (see MF_01258), as well as some eubacteria such as Streptomyces, Mycobacterium, Nocardia, and Thermobifida (this rule, MF_01259). The length of the polyglutamate tail varies in different organisms. According to PubMed:12867481 (Graupner et al., 2003) and PubMed:11479701 (Bair et al., 2001): Analyses of the F420s present in Methanococcus jannaschii have shown that these cells contain a series of gamma-glutamyl-linked F420s capped with a single, terminal alpha-linked L-glutamate. The predominant form of F420 was designated as alpha-F420-3 and represented 86% of the F420s in these cells. Analyses of Methanosarcina thermophila, Methanosarcina barkeri, Methanobacterium thermoautotrophicum, Archaeoglobus fulgidus, and Mycobacterium smegmatis showed that they contained only gamma-glutamyl-linked F420s, with the following predominant forms: Methanosarcina thermophila (gamma-F420-3, 19%; gamma-F420-4, 62%), Methanosarcina barkeri (gamma-F420-2, 31%; gamma-F420-4, 32%), Methanobacterium thermoautotrophicum (gamma-F420-2, 80%), Archaeoglobus fulgidus (gamma-F420-4, 89%), and Mycobacterium smegmatis (gamma-F420-5, 67%; gamma-F420-6, 31%). In mycobacteria, the length of the polyglutamate tail varies by up to nine residues, with predominant species containing four to seven glutamate molecules (Bair et al., 2001, PubMed:11479701; Bashiri et al., 2008, PubMed:18434308).


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