AC MF_01336; DC Protein; auto TR HAMAP; MF_01336; -; 1; level=0 XX Names: Ribosomal_bL25 XX ID RL25 DE RecName: Full=Large ribosomal subunit protein bL25; GN Name=rplY; XX CC -!- FUNCTION: This is one of the proteins that binds to the 5S RNA in the CC ribosome where it forms part of the central protuberance. CC -!- SUBUNIT: Part of the 50S ribosomal subunit; part of the 5S CC rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA CC independently of L5 and L18. CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL25 family. XX DR Pfam; PF01386; Ribosomal_L25p; 1; trigger=no DR PRINTS; PR00453; VWFADOMAIN; 1; trigger=no XX KW Ribonucleoprotein KW Ribosomal protein KW RNA-binding KW rRNA-binding XX GO GO:0003723; F:RNA binding GO GO:0006412; P:translation XX FT None XX Size: 92-103; Related: MF_01334!; Template: P68919; Q5SHZ1; P56930; Scope: Bacteria Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: This family is specific for proteins with only the L25 domain. This protein varies in length; most bacteria encode both the L25 and CTC domains (see MF_01334). However a number of bacteria, including some Gammaproteobacteria, Cyanobacteriota and at least one Betaproteobacteria, have only the L25 domain (this family), while A. aeolicus only has the CTC domain (and cannot bind 5S rRNA). In at least some cases bL25 is dispensible for translation; in B. subtilis bL25 is only expressed in vegetatively growing cells. bL25 is missing from some Bacillus, Cyanobacteriota and Lactobacillus. The equivalent of the highly conserved Asp-87 in Thermus thermophilus (AC P56930) is altered in Enterococcus and Cyanobacteriota. There are compensatory changes in the 5S rRNA that are thought to alter its secondary structure and thus permit binding of the protein to rRNA, see: PubMed=15718233; DOI=10.1074/jbc.M413596200; Gongadze G.M., Korepanov A.P., Stolboushkina E.A., Zelinskaya N.V., Korobeinikova A.V., Ruzanov M.V., Eliseev B.D., Nikonov O.S., Nikonov S.V., Garber M.B., Lim V.I.; "The crucial role of conserved intermolecular H-bonds inaccessible to the solvent in formation and stabilization of the TL5.5 SrRNA complex."; J. Biol. Chem. 280:16151-16156(2005). XX # Revision 1.17 2023/09/22 //