HAMAP rule MF_03215

General rule information [?]

Accession	MF_03215
Dates	29-FEB-2016 (Created) 1-JUN-2023 (Last updated, Version 9)
Name	Ribokinase_euk
Scope(s)	Eukaryota
Template(s)	Q9H477 (RBSK_HUMAN); P0A9J6 (RBSK_ECOLI); [ Recover all ]
Triggered by	case c? <OC:Eukaryota> HAMAP; MF_01987 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	RBSK
Protein name	RecName: Full=Ribokinase; Short=RK; EC=2.7.1.15;
case <OC:Vertebrata>
Gene name	Name=RBKS;
else case <OC:Saccharomycotina>
Gene name	Name=RBK1;
end case

Comments [?]

FUNCTION	Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.
CATALYTIC ACTIVITY	Reaction=ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+); Xref=Rhea:RHEA:13697, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:47013, ChEBI:CHEBI:78346, ChEBI:CHEBI:456216; EC=2.7.1.15;
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.;
ACTIVITY REGULATION	Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.
PATHWAY	Carbohydrate metabolism; D-ribose degradation; D-ribose 5- phosphate from beta-D-ribopyranose: step 2/2.
SUBUNIT	Homodimer.
SUBCELLULAR LOCATION	Cytoplasm. Nucleus.
SIMILARITY	Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.

Keywords [?]

ATP-binding

Carbohydrate metabolism

Gene Ontology [?]

GO:0005737; Cellular component:cytoplasm

GO:0005634; Cellular component:nucleus

GO:0005524; Molecular function:ATP binding

GO:0004747; Molecular function:ribokinase activity

GO:0046835; Biological process:carbohydrate phosphorylation

GO:0019303; Biological process:D-ribose catabolic process

Cross-references [?]

PROSITE	PS00584; PFKB_KINASES_2; 1;
Pfam	PF00294; PfkB; 1;
PRINTS	PR00990; RIBOKINASE; 1;
NCBIfam	TIGR02152; D_ribokin_bact; 1;

Features [?]

From: RBSK_HUMAN (Q9H477)

Key

From

Description

Tag

Condition

FTGroup

BINDING

235

240

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

x(2)-G-x(2)-G

BINDING

268

269

/ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"

G-D

BINDING

/ligand="substrate"

x(2)-D

BINDING

/ligand="substrate"

G-[KR]-[GAS]-x-[NR]

ACT_SITE

269

/note="Proton acceptor"

BINDING

263