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HAMAP rule MF_03221

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General rule information [?]

Accession MF_03221
Dates 20-OCT-2016 (Created)
3-SEP-2024 (Last updated, Version 10)
Name Succ_CoA_betaG_euk
Scope(s) Eukaryota
Template(s) P53590 (SUCB2_PIG); [ Recover all ]
Triggered by
case c? <OC:Metazoa> or <OC:Amoebozoa>
HAMAP; MF_03221 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SUCB2
Protein name RecName: Full=Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial;
                 EC=6.2.1.4;
AltName: Full=GTP-specific succinyl-CoA synthetase subunit beta;
                 Short=G-SCS;
                 Short=GTPSCS;
AltName: Full=Succinyl-CoA synthetase beta-G chain;
                 Short=SCS-betaG;
case <OC:Vertebrata>
Gene name Name=SUCLG2;
end case

Comments [?]

FUNCTIONGTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
CATALYTIC ACTIVITY Reaction=GTP + succinate + CoA = succinyl-CoA + GDP + phosphate; Xref=Rhea:RHEA:22120, ChEBI:CHEBI:30031, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292, ChEBI:CHEBI:58189; EC=6.2.1.4;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
PATHWAYCarbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
SUBUNITHeterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.
SUBCELLULAR LOCATIONMitochondrion.
SIMILARITYBelongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.
case not <AnyFeature:TransitM>
MISCELLANEOUSThis protein may be expected to contain an N-terminal transit peptide but none has been predicted.
end case

Keywords [?]

GTP-binding
Ligase
case <FTGroup:1>
Magnesium
Metal-binding
end case
Mitochondrion
Nucleotide-binding
Tricarboxylic acid cycle

Gene Ontology [?]

GO:0005525; Molecular function:GTP binding
case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0004775; Molecular function:succinate-CoA ligase (ADP-forming) activity
GO:0006099; Biological process:tricarboxylic acid cycle

Cross-references [?]

PROSITE PS01217; SUCCINYL_COA_LIG_3; 1;
PROSITE PS50975; ATP_GRASP; 1;
Pfam PF08442; ATP-grasp_2; 1;
Pfam PF00549; Ligase_CoA; 1;
NCBIfam TIGR01016; sucCoAbeta; 1;
PIRSF PIRSF001554; SucCS_beta; 1;
General TransitM; -; 0-1;

Features [?]

From: SUCB2_PIG (P53590)
Key From To Description Tag Condition FTGroup
case <Feature:PS50975>
DOMAIN 47 275 /note="ATP-grasp"
end case
BINDING 91 93 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 G-R-G
BINDING 366 368 /ligand="substrate"
/ligand_note="ligand shared with subunit alpha"		 G-I-[VM]
BINDING 244 244 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 N 1
BINDING 258 258 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D 1
BINDING 58 58 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"		 Q
BINDING 147 147 /ligand="GTP"
/ligand_id="ChEBI:CHEBI:37565"
BINDING 309 309 /ligand="substrate"
/ligand_note="ligand shared with subunit alpha"		 N
SITE 80 80 /note="Important for substrate specificity" E
SITE 148 148 /note="Important for substrate specificity" [DN]

Additional information [?]

Size range 367-542 amino acids
Related rules MF_00558
MF_03220
Fusion Nter: None Cter: None
Comments In animals, two different isoforms of SCS exist, one specific for ATP and the other specific for GTP (PubMed:9765291). The two isoforms include the same alpha-subunit, but different beta-subunits (PubMed:9765290). This is the subfamily of GTP-specific beta subunits.



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