AC MF_00016; DC Protein; auto TR HAMAP; MF_00016; -; 1; level=0 XX Names: DNA_HJ_migration_RuvB XX ID RUVB DE RecName: Full=Holliday junction branch migration complex subunit RuvB; DE EC=3.6.4.12; GN Name=ruvB; XX CC -!- FUNCTION: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) CC DNA during genetic recombination and DNA repair, while the RuvA-RuvB CC complex plays an important role in the rescue of blocked DNA CC replication forks via replication fork reversal (RFR). RuvA CC specifically binds to HJ cruciform DNA, conferring on it an open CC structure. The RuvB hexamer acts as an ATP-dependent pump, pulling CC dsDNA into and through the RuvAB complex. RuvB forms 2 homohexamers on CC either side of HJ DNA bound by 1 or 2 RuvA tetramers; 4 subunits per CC hexamer contact DNA at a time. Coordinated motions by a converter CC formed by DNA-disengaged RuvB subunits stimulates ATP hydrolysis and CC nucleotide exchange. Immobilization of the converter enables RuvB to CC convert the ATP-contained energy into a lever motion, pulling 2 CC nucleotides of DNA out of the RuvA tetramer per ATP hydrolyzed, thus CC driving DNA branch migration. The RuvB motors rotate together with the CC DNA substrate, which together with the progressing nucleotide cycle CC form the mechanistic basis for DNA recombination by continuous HJ CC branch migration. Branch migration allows RuvC to scan DNA until it CC finds its consensus sequence, where it cleaves and resolves cruciform CC DNA. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CC -!- SUBUNIT: Homohexamer. Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ) CC complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters CC through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA CC strand where it exits the tetramer. Each RuvB hexamer is contacted by CC two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this CC complex drives branch migration. In the full resolvosome a probable CC DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- DOMAIN: Has 3 domains, the large (RuvB-L) and small ATPase (RuvB-S) CC domains and the C-terminal head (RuvB-H) domain. The head domain binds CC DNA, while the ATPase domains jointly bind ATP, ADP or are empty CC depending on the state of the subunit in the translocation cycle. CC During a single DNA translocation step the structure of each domain CC remains the same, but their relative positions change. CC -!- SIMILARITY: Belongs to the RuvB family. XX DR Pfam; PF17864; AAA_lid_4; 1; trigger=no DR Pfam; PF05491; RuvB_C; 1; trigger=no DR Pfam; PF05496; RuvB_N; 1; trigger=no DR NCBIfam; TIGR00635; RuvB; 1; trigger=no XX KW Cytoplasm KW DNA damage KW DNA repair case KW SOS response end case KW DNA-binding KW ATP-binding KW Nucleotide-binding KW DNA recombination KW Hydrolase XX GO GO:0005524; F:ATP binding GO GO:0000400; F:four-way junction DNA binding GO GO:0048476; C:Holliday junction resolvase complex GO GO:0006310; P:DNA recombination GO GO:0006281; P:DNA repair case GO GO:0009432; P:SOS response end case XX FT From: RUVB_STRT2 (Q5M2B1) FT REGION 1..181 FT /note="Large ATPase domain (RuvB-L)" FT REGION 182..252 FT /note="Small ATPAse domain (RuvB-S)" FT REGION 255..Cter FT /note="Head domain (RuvB-H)" FT BINDING 20 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: [LI] FT BINDING 21 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: R FT BINDING 62 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: G FT BINDING 65 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: K FT BINDING 66 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: T FT BINDING 66 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT Condition: T FT BINDING 67 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: [TS] FT BINDING 128..130 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: [ED]-D-[YF] FT BINDING 171 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: R FT BINDING 181 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: Y FT BINDING 218 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: R FT BINDING 291 FT /ligand="DNA" FT /ligand_id="ChEBI:CHEBI:16991" FT Optional; Condition: R FT BINDING 310 FT /ligand="DNA" FT /ligand_id="ChEBI:CHEBI:16991" FT Optional; Condition: [RK] FT BINDING 312 FT /ligand="DNA" FT /ligand_id="ChEBI:CHEBI:16991" FT Optional; Condition: R FT BINDING 315 FT /ligand="DNA" FT /ligand_id="ChEBI:CHEBI:16991" FT Optional; Condition: R XX Size: 290-415; Related: None; Template: P0A812; Q5SL87; Q5M2B1; Scope: Bacteria Archaea; Methanomicrobiales Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.34 2023/06/01 //