AC MF_00021; DC Protein; auto TR HAMAP; MF_00021; -; 1; level=0 XX Names: ThiI XX ID THII case DE RecName: Full=tRNA sulfurtransferase; DE EC=2.8.1.4; DE AltName: Full=Sulfur carrier protein ThiS sulfurtransferase; DE AltName: Full=Thiamine biosynthesis protein ThiI; DE AltName: Full=tRNA 4-thiouridine synthase; end case case not DE RecName: Full=Probable tRNA sulfurtransferase; DE EC=2.8.1.4; DE AltName: Full=Sulfur carrier protein ThiS sulfurtransferase; DE AltName: Full=Thiamine biosynthesis protein ThiI; DE AltName: Full=tRNA 4-thiouridine synthase; end case GN Name=thiI; XX CC -!- FUNCTION: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to CC produce 4-thiouridine in position 8 of tRNAs, which functions as a CC near-UV photosensor. Also catalyzes the transfer of sulfur to the CC sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a CC step in the synthesis of thiazole, in the thiamine biosynthesis CC pathway. The sulfur is donated as persulfide by IscS. CC -!- CATALYTIC ACTIVITY: CC Reaction=[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a CC uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = CC [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + CC AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]; CC Xref=Rhea:RHEA:24176, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, CC Rhea:RHEA-COMP:13337, Rhea:RHEA-COMP:13338, Rhea:RHEA-COMP:13339, CC Rhea:RHEA-COMP:13340, ChEBI:CHEBI:15378, ChEBI:CHEBI:29950, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33737, CC ChEBI:CHEBI:33738, ChEBI:CHEBI:61963, ChEBI:CHEBI:65315, CC ChEBI:CHEBI:136798, ChEBI:CHEBI:456215; EC=2.8.1.4; CC -!- CATALYTIC ACTIVITY: CC Reaction=[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + CC S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier CC protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L- CC cysteinyl-[cysteine desulfurase]; Xref=Rhea:RHEA:43340, Rhea:RHEA- CC COMP:12157, Rhea:RHEA-COMP:12158, Rhea:RHEA-COMP:12908, Rhea:RHEA- CC COMP:12910, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, CC ChEBI:CHEBI:29950, ChEBI:CHEBI:61963, ChEBI:CHEBI:90618, CC ChEBI:CHEBI:90619, ChEBI:CHEBI:456215; CC -!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: Belongs to the ThiI family. XX DR PROSITE; PS50206; RHODANESE_3; 0-1; trigger=yes DR PROSITE; PS51165; THUMP; 1; trigger=yes DR Pfam; PF02568; ThiI; 1; trigger=no DR Pfam; PF02926; THUMP; 1; trigger=no DR Pfam; PF00581; Rhodanese; 1; trigger=no DR NCBIfam; TIGR04271; ThiI_C_thiazole; 0-1; trigger=no DR NCBIfam; TIGR00342; TIGR00342; 1; trigger=no XX case KW RNA-binding KW tRNA-binding end case KW ATP-binding KW Cytoplasm case KW Disulfide bond KW Redox-active center end case KW Nucleotide-binding KW Thiamine biosynthesis KW Transferase XX case GO GO:0000049; F:tRNA binding end case GO GO:0005524; F:ATP binding GO GO:0016783; F:sulfurtransferase activity GO GO:0009228; P:thiamine biosynthetic process GO GO:0034227; P:tRNA thio-modification GO GO:0005737; C:cytoplasm XX FT From: THII_BACAN (Q81KU0) FT BINDING 183..184 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: [LM]-x FT BINDING 208..209 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Optional; Condition: [HYNTS]-[FY] FT BINDING 265 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: [RK] FT BINDING 287 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: G FT BINDING 296 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: Q FT From: THII_ECOLI (P77718) case FT ACT_SITE 456 FT /note="Cysteine persulfide intermediate" FT Condition: C FT DISULFID 344..456 FT /note="Redox-active" FT Condition: C-x*-C end case XX Size: 368-486; Related: None; Template: P77718; P55913; Q81KU0; Scope: Bacteria Archaea Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: Some proteins of this family do not possess a rhodanese domain, so there must be an external rhodanese-like partner involved. It is not known, and the reaction has not been characterized in this case. XX # Revision 1.43 2023/06/01 //