AC   MF_00022;
DC   Protein; auto
TR   HAMAP; MF_00022; -; 1; level=0
XX
Names: Glu_tRNA_synth_type1
XX
ID   SYE
DE   RecName: Full=Glutamate--tRNA ligase;
DE            EC=6.1.1.17;
DE   AltName: Full=Glutamyl-tRNA synthetase;
DE            Short=GluRS;
GN   Name=gltX;
XX
CC   -!- FUNCTION: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-
CC       step reaction: glutamate is first activated by ATP to form Glu-AMP and
CC       then transferred to the acceptor end of tRNA(Glu).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-
CC         glutamyl-tRNA(Glu); Xref=Rhea:RHEA:23540, Rhea:RHEA-COMP:9663,
CC         Rhea:RHEA-COMP:9680, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:78442, ChEBI:CHEBI:78520,
CC         ChEBI:CHEBI:456215; EC=6.1.1.17;
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC       Note=Binds 1 zinc ion per subunit.;
end case
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       Glutamate--tRNA ligase type 1 subfamily.
XX
DR   Pfam; PF00749; tRNA-synt_1c; 1; trigger=no
DR   PRINTS; PR00987; TRNASYNTHGLU; 1; trigger=no
DR   NCBIfam; TIGR00464; GltX_bact; 1; trigger=no
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1; trigger=no
XX
KW   Aminoacyl-tRNA synthetase
KW   ATP-binding
KW   Cytoplasm
KW   Ligase
KW   Nucleotide-binding
KW   Protein biosynthesis
case <FTGroup:1>
KW   Metal-binding
KW   Zinc
end case
XX
GO   GO:0005524; F:ATP binding
GO   GO:0004818; F:glutamate-tRNA ligase activity
case <FTGroup:1>
GO   GO:0008270; F:zinc ion binding
end case
GO   GO:0006424; P:glutamyl-tRNA aminoacylation
GO   GO:0005737; C:cytoplasm
XX
FT   From: SYE_ECOLI (P04805)
FT   MOTIF           9..19
FT                   /note="'HIGH' region"
FT   Condition: P-[SN]-P-x(4)-[HR]-[LIVMAT]-[GE]-x
FT   MOTIF           237..241
FT                   /note="'KMSKS' region"
FT   Condition: x-[LIMSF]-S-K-[KR]
FT   BINDING         98
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Optional; Group: 1; Condition: C
FT   BINDING         100
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Optional; Group: 1; Condition: C
FT   BINDING         125
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Optional; Group: 1; Condition: [CH]
FT   BINDING         127
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Optional; Group: 1; Condition: [HED]
FT   BINDING         240
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: K
XX
Size: 431-546;
Related: MF_01428;
Template: P04805; O25360; P27000; Q8DLI5;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: <Unknown>
Duplicate: None
Plasmid: None
Comments: This signature is specific for bacterial and eukaryotic mitochondrial glutamate--tRNA ligases.
 See MF_02076 for archaeal and eukaryotic cytoplasmic glutamate--tRNA ligases.
XX
# Revision 1.50 2023/06/01
//