AC   MF_00061;
DC   Protein; auto
TR   HAMAP; MF_00061; -; 1; level=0
XX
Names: IspE
XX
ID   ISPE
case <OC:Staphylococcus> or <OC:Streptococcus>
DE   RecName: Full=Putative 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase;
DE            Short=CMK;
DE            EC=2.7.1.148;
DE   AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;
else
DE   RecName: Full=4-diphosphocytidyl-2-C-methyl-D-erythritol kinase;
DE            Short=CMK;
DE            EC=2.7.1.148;
DE   AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;
GN   Name=ispE;
end case
XX
CC   -!- FUNCTION: Catalyzes the phosphorylation of the position 2 hydroxy group
CC       of 4-diphosphocytidyl-2C-methyl-D-erythritol.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-
CC         erythritol 2-phosphate + ADP + H(+); Xref=Rhea:RHEA:18437,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57823,
CC         ChEBI:CHEBI:57919, ChEBI:CHEBI:456216; EC=2.7.1.148;
case not <OC:Staphylococcus> and not <OC:Streptococcus>
CC   -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis
CC       via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-
CC       phosphate: step 3/6.
end case
case <OC:Enterobacterales>
CC   -!- SUBUNIT: Homodimer.
end case
CC   -!- SIMILARITY: Belongs to the GHMP kinase family. IspE subfamily.
XX
DR   Pfam; PF08544; GHMP_kinases_C; 1; trigger=no
DR   Pfam; PF00288; GHMP_kinases_N; 1; trigger=no
DR   PIRSF; PIRSF010376; IspE; 1; trigger=no
DR   NCBIfam; TIGR00154; IspE; 1; trigger=no
XX
KW   ATP-binding
KW   Nucleotide-binding
case not <OC:Staphylococcus> and not <OC:Streptococcus>
KW   Isoprene biosynthesis
end case
KW   Kinase
KW   Transferase
XX
GO   GO:0050515; F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity
GO   GO:0005524; F:ATP binding
case not <OC:Staphylococcus> and not <OC:Streptococcus>
GO   GO:0016114; P:terpenoid biosynthetic process
end case
XX
FT   From: ISPE_ECOL6 (Q8FI04)
FT   BINDING         99..109
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: [PL]-x-x-[GSA]-G-[LIMFV]-[GA]-[GS]-[GAD]-S-[STAG]
FT   ACT_SITE        10
FT   Condition: K
FT   ACT_SITE        141
FT   Condition: D
XX
Size: 255-329;
Related: None;
Template: P62615; Q8FI04; P83700;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: In CHLPN there is a remnant of an ispE gene but which is interrupted by a
 probable natural frameshift (all CHLPN strains have it). It is orf CP0905
XX
# Revision 1.36 2023/06/01
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