AC   MF_00062;
DC   Protein; auto
TR   HAMAP; MF_00062; -; 1; level=0
XX
Names: Sulf_adenylyltr_sub1
XX
ID   CYSN
DE   RecName: Full=Sulfate adenylyltransferase subunit 1;
DE            EC=2.7.7.4;
DE   AltName: Full=ATP-sulfurylase large subunit;
DE   AltName: Full=Sulfate adenylate transferase;
DE            Short=SAT;
GN   Name=cysN;
XX
CC   -!- FUNCTION: With CysD forms the ATP sulfurylase (ATPS) that catalyzes the
CC       adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and
CC       diphosphate, the first enzymatic step in sulfur assimilation pathway.
CC       APS synthesis involves the formation of a high-energy phosphoric-
CC       sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled
CC       to ATP hydrolysis by CysD.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H(+) + sulfate = adenosine 5'-phosphosulfate +
CC         diphosphate; Xref=Rhea:RHEA:18133, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16189, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58243; EC=2.7.7.4;
CC   -!- PATHWAY: Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from
CC       sulfate: step 1/3.
CC   -!- SUBUNIT: Heterodimer composed of CysD, the smaller subunit, and CysN.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. CysN/NodQ
CC       subfamily.
XX
DR   Pfam; PF00009; GTP_EFTU; 1; trigger=no
DR   PRINTS; PR00315; ELONGATNFCT; 1; trigger=no
DR   PROSITE; PS00301; G_TR_1; 1; trigger=no
DR   PROSITE; PS51722; G_TR_2; 1; trigger=no
XX
KW   ATP-binding
KW   GTP-binding
KW   Nucleotide-binding
KW   Nucleotidyltransferase
KW   Transferase
XX
GO   GO:0005525; F:GTP binding
GO   GO:0004781; F:sulfate adenylyltransferase (ATP) activity
GO   GO:0000103; P:sulfate assimilation
XX
FT   From: CYSN_ECOLI (P23845)
FT   BINDING         34..41
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: G-x(4)-G-K-S
FT   BINDING         113..117
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: D-[ATC]-P-G-H
FT   BINDING         168..171
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: N-K-[IM]-D
XX
Size: 441-494;
Related: None;
Template: P23845;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: MF_00065 (cysC)
Duplicate: None
Plasmid: in AZOBR, RHIME
Comments: Problematic entry.
 N-terminal too short in MYCTU.
 If in a nodulating bacteria, has to be done manually because of the
 presence of both cysN and nodQ.
XX
# Revision 1.31 2022/11/19
//