AC   MF_00071;
DC   Protein; auto
c?   <OC:Bacteria>
TR   HAMAP; MF_00071; -; 1; level=0
XX
Names: LepA
XX
ID   LEPA
DE   RecName: Full=Elongation factor 4;
DE            Short=EF-4;
DE            EC=3.6.5.n1;
DE   AltName: Full=Ribosomal back-translocase LepA;
GN   Name=lepA;
XX
CC   -!- FUNCTION: Required for accurate and efficient protein synthesis under
CC       certain stress conditions. May act as a fidelity factor of the
CC       translation reaction, by catalyzing a one-codon backward translocation
CC       of tRNAs on improperly translocated ribosomes. Back-translocation
CC       proceeds from a post-translocation (POST) complex to a pre-
CC       translocation (PRE) complex, thus giving elongation factor G a second
CC       chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-
CC       dependent manner.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.n1;
case not defined <Property:Membrane> or <Property:Membrane=1>
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein;
CC       Cytoplasmic side.
else case <Property:Membrane=2>
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein;
CC       Cytoplasmic side.
end case
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. LepA subfamily.
XX
DR   Pfam; PF00679; EFG_C; 1; trigger=no
DR   Pfam; PF00009; GTP_EFTU; 1; trigger=no
DR   Pfam; PF03144; GTP_EFTU_D2; 1; trigger=no
DR   Pfam; PF06421; LepA_C; 1; trigger=no
DR   PRINTS; PR00315; ELONGATNFCT; 1; trigger=no
DR   NCBIfam; TIGR01393; LepA; 1; trigger=no
DR   NCBIfam; TIGR00231; Small_GTP; 1; trigger=no
DR   PROSITE; PS00301; G_TR_1; 1; trigger=no
DR   PROSITE; PS51722; G_TR_2; 1; trigger=no
XX
KW   Cell membrane
case defined <Property:Membrane> and <Property:Membrane=2>
KW   Cell inner membrane
end case
KW   GTP-binding
KW   Hydrolase
KW   Membrane
KW   Nucleotide-binding
KW   Protein biosynthesis
XX
GO   GO:0005886; C:plasma membrane
GO   GO:0005525; F:GTP binding
GO   GO:0003924; F:GTPase activity
GO   GO:0043022; F:ribosome binding
GO   GO:0003746; F:translation elongation factor activity
GO   GO:0045727; P:positive regulation of translation
XX
FT   From: LEPA_AQUAE (O67618)
FT   BINDING         16..21
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: [DN]-H-G-K-[ST]-T
FT   BINDING         133..136
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: [NS]-K-[LIVMCA]-D
XX
Size: 593-660;
Related: MF_03138!;
Template: P60785; O67618; Q2FXY7;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: in LACPL, METFK, RHOBA
Plasmid: None
Comments: None
XX
# Revision 1.30 2023/06/01
//