AC   MF_00086;
DC   Protein; auto
TR   HAMAP; MF_00086; -; 1; level=0
XX
Names: S_AdoMet_synth1
XX
ID   METK
DE   RecName: Full=S-adenosylmethionine synthase;
DE            Short=AdoMet synthase;
DE            EC=2.5.1.6;
DE   AltName: Full=MAT;
DE   AltName: Full=Methionine adenosyltransferase;
GN   Name=metK;
XX
CC   -!- FUNCTION: Catalyzes the formation of S-adenosylmethionine (AdoMet) from
CC       methionine and ATP. The overall synthetic reaction is composed of two
CC       sequential steps, AdoMet formation and the subsequent tripolyphosphate
CC       hydrolysis which occurs prior to release of AdoMet from the enzyme.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + L-methionine = diphosphate + phosphate + S-
CC         adenosyl-L-methionine; Xref=Rhea:RHEA:21080, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:59789; EC=2.5.1.6;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Note=Binds 2 divalent ions per subunit.;
CC   -!- COFACTOR:
CC       Name=K(+); Xref=ChEBI:CHEBI:29103;
CC       Note=Binds 1 potassium ion per subunit.;
CC   -!- PATHWAY: Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis;
CC       S-adenosyl-L-methionine from L-methionine: step 1/1.
CC   -!- SUBUNIT: Homotetramer; dimer of dimers.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the AdoMet synthase family.
XX
DR   Pfam; PF02773; S-AdoMet_synt_C; 1; trigger=no
DR   Pfam; PF02772; S-AdoMet_synt_M; 1; trigger=no
DR   Pfam; PF00438; S-AdoMet_synt_N; 1; trigger=no
DR   PIRSF; PIRSF000497; MAT; 1; trigger=no
DR   NCBIfam; TIGR01034; metK; 1; trigger=no
DR   PROSITE; PS00376; ADOMET_SYNTHASE_1; 1; trigger=no
DR   PROSITE; PS00377; ADOMET_SYNTHASE_2; 1; trigger=no
XX
KW   ATP-binding
KW   Cytoplasm
KW   Magnesium
KW   Metal-binding
KW   Nucleotide-binding
KW   One-carbon metabolism
KW   Potassium
KW   Transferase
XX
GO   GO:0005524; F:ATP binding
GO   GO:0000287; F:magnesium ion binding
GO   GO:0004478; F:methionine adenosyltransferase activity
GO   GO:0006730; P:one-carbon metabolic process
GO   GO:0005737; C:cytoplasm
XX
FT   From: METK_ECOLI (P0A817)
FT   BINDING         164..166
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT                   /note="in other chain"
FT   Optional; Condition: D-x-K
FT   BINDING         230..231
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT                   /note="in other chain"
FT   Optional; Condition: [RK]-F
FT   BINDING         245..246
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT                   /note="in other chain"
FT   Optional; Condition: R-K
FT   REGION          99..109
FT                   /note="Flexible loop"
FT   BINDING         17
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: D
FT   BINDING         43
FT                   /ligand="K(+)"
FT                   /ligand_id="ChEBI:CHEBI:29103"
FT   Condition: E
FT   BINDING         15
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT                   /note="in other chain"
FT   Optional; Condition: H
FT   BINDING         56
FT                   /ligand="L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:57844"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT                   /note="in other chain"
FT   Optional; Condition: E
FT   BINDING         99
FT                   /ligand="L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:57844"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT                   /note="in other chain"
FT   Optional; Condition: Q
FT   BINDING         239
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT   Optional; Condition: D
FT   BINDING         239
FT                   /ligand="L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:57844"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT   Optional; Condition: D
FT   BINDING         262
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT   Optional; Condition: [AS]
FT   BINDING         266
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT   Optional; Condition: K
FT   BINDING         270
FT                   /ligand="L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:57844"
FT                   /ligand_note="ligand shared between two neighboring
FT                   subunits"
FT                   /note="in other chain"
FT   Optional; Condition: K
XX
Size: 376-430;
Related: None;
Template: P0A817;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: in FRACC, RHOPB, RHORT
Plasmid: None
Comments: None
XX
# Revision 1.48 2023/06/01
//