AC   MF_00092;
DC   Protein; auto
TR   HAMAP; MF_00092; -; 1; level=0
XX
Names: MutS2
XX
ID   MUTS2
DE   RecName: Full=Endonuclease MutS2;
DE            EC=3.1.-.-;
DE   AltName: Full=Ribosome-associated protein quality control-upstream factor;
DE            Short=RQC-upstream factor;
DE            Short=RqcU;
DE            EC=3.6.4.-;
GN   Name=mutS2; Synonyms=rqcU;
XX
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and thus may have a key role in the control of
CC       bacterial genetic diversity.
CC   -!- FUNCTION: Acts as a ribosome collision sensor, splitting the ribosome
CC       into its 2 subunits. Detects stalled/collided 70S ribosomes which it
CC       binds and splits by an ATP-hydrolysis driven conformational change.
CC       Acts upstream of the ribosome quality control system (RQC), a ribosome-
CC       associated complex that mediates the extraction of incompletely
CC       synthesized nascent chains from stalled ribosomes and their subsequent
CC       degradation. Probably generates substrates for RQC.
CC   -!- SUBUNIT: Homodimer. Binds to stalled ribosomes, contacting rRNA.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily.
XX
DR   Pfam; PF00488; MutS_V; 1; trigger=no
DR   Pfam; PF01713; Smr; 1; trigger=no
DR   PIRSF; PIRSF005814; MutS_YshD; 1; trigger=no
DR   NCBIfam; TIGR01069; MutS2; 1; trigger=no
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1; trigger=no
DR   PROSITE; PS50828; SMR; 1; trigger=yes
XX
KW   ATP-binding
KW   DNA-binding
KW   Endonuclease
KW   Hydrolase
KW   Nuclease
KW   Nucleotide-binding
KW   RNA-binding
KW   rRNA-binding
XX
GO   GO:0005524; F:ATP binding
GO   GO:0003677; F:DNA binding
GO   GO:0004519; F:endonuclease activity
GO   GO:0072344; P:rescue of stalled ribosome
GO   GO:0019843; F:rRNA binding
GO   GO:0043023; F:ribosomal large subunit binding
XX
FT   From: MUTS2_THET8 (Q5SHT5)
FT   BINDING         315..322
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: G-x-N-x-G-G-K-[TS]
XX
Size: 660-920;
Related: None;
Template: Q5SHT5; O25338; Q9X105;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: Endonuclease activity has been proven for T.thermophilus (Q5SHT5), T.
 maritima (Q9X105), D.radiodurans (Q9RSZ3) but could not be demonstrated in B.
 subtilis (P94545).
XX
# Version: 21
# Last updated date: 2024-09-02
# Created date: 2005-02-28
//