AC   MF_00107;
DC   Protein; auto
TR   HAMAP; MF_00107; -; 1; level=0
XX
Names: IspF
XX
ID   ISPF
DE   RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase;
DE            Short=MECPP-synthase;
DE            Short=MECDP-synthase;
DE            Short=MECPS;
DE            EC=4.6.1.12;
GN   Name=ispF;
XX
CC   -!- FUNCTION: Involved in the biosynthesis of isopentenyl diphosphate (IPP)
CC       and dimethylallyl diphosphate (DMAPP), two major building blocks of
CC       isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-
CC       C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol
CC       2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine
CC       5-monophosphate (CMP).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-
CC         erythritol 2,4-cyclic diphosphate + CMP; Xref=Rhea:RHEA:23864,
CC         ChEBI:CHEBI:57919, ChEBI:CHEBI:58483, ChEBI:CHEBI:60377; EC=4.6.1.12;
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC       Note=Binds 1 divalent metal cation per subunit.;
CC   -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis
CC       via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-
CC       phosphate: step 4/6.
CC   -!- SUBUNIT: Homotrimer.
CC   -!- SIMILARITY: Belongs to the IspF family.
XX
DR   Pfam; PF02542; YgbB; 1; trigger=no
DR   NCBIfam; TIGR00151; IspF; 1; trigger=no
DR   PROSITE; PS01350; ISPF; 1; trigger=no
XX
KW   Isoprene biosynthesis
KW   Lyase
KW   Metal-binding
XX
GO   GO:0008685; F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
GO   GO:0016114; P:terpenoid biosynthetic process
XX
FT   From: ISPF_ECOLI (P62617)
FT   BINDING         8..10
FT                   /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57919"
FT   Condition: D-x-H
FT   BINDING         34..35
FT                   /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57919"
FT   Condition: H-S
FT   BINDING         56..58
FT                   /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57919"
FT   Condition: [DNEK]-[IVL]-[GA]
FT   BINDING         61..65
FT                   /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57919"
FT   Condition: F-x-[DNEQ]-x-[DNEQ]
FT   BINDING         100..106
FT                   /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57919"
FT   Condition: [ALVI]-x(2)-P-[KNR]-M-[LAGI]
FT   BINDING         132..135
FT                   /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57919"
FT   Condition: [TS]-[TS]-x-[EDN]
FT   BINDING         8
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Condition: D
FT   BINDING         10
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Condition: H
FT   BINDING         42
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT   Condition: H
FT   BINDING         139
FT                   /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57919"
FT   Condition: F
FT   BINDING         142
FT                   /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57919"
FT   Condition: [RHK]
FT   SITE            34
FT                   /note="Transition state stabilizer"
FT   Condition: [HDN]
FT   SITE            133
FT                   /note="Transition state stabilizer"
FT   Condition: [TS]
XX
Size: 152-184;
Related: MF_01520!;
Template: P62617; P44815; Q8RQP5; P9WKG5; Q63T71; Q3JRA0; Q06756; Q5NFU1; Q8ZMF7; Q8EBR3; Q8ZBP7;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: See MF_01520 for bifunctional ispDF
XX
# Revision 1.40 2023/06/01
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