AC MF_00108; DC Protein; auto TR HAMAP; MF_00108; -; 1; level=0 XX Names: IspD XX ID ISPD DE RecName: Full=2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; DE EC=2.7.7.60; DE AltName: Full=4-diphosphocytidyl-2C-methyl-D-erythritol synthase; DE AltName: Full=MEP cytidylyltransferase; DE Short=MCT; GN Name=ispD; XX CC -!- FUNCTION: Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D- CC erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). CC -!- CATALYTIC ACTIVITY: CC Reaction=2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C- CC methyl-D-erythritol + diphosphate; Xref=Rhea:RHEA:13429, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, CC ChEBI:CHEBI:57823, ChEBI:CHEBI:58262; EC=2.7.7.60; CC -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis CC via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- CC phosphate: step 2/6. case CC -!- SUBUNIT: Homodimer. end case CC -!- SIMILARITY: Belongs to the IspD/TarI cytidylyltransferase family. IspD CC subfamily. XX DR Pfam; PF01128; IspD; 1; trigger=no DR NCBIfam; TIGR00453; IspD; 1; trigger=no DR PROSITE; PS01295; ISPD; 1; trigger=no XX KW Isoprene biosynthesis KW Nucleotidyltransferase KW Transferase XX GO GO:0050518; F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity GO GO:0016114; P:terpenoid biosynthetic process XX FT From: ISPD_ECOLI (Q46893) FT SITE 20 FT /note="Transition state stabilizer" FT Condition: R FT SITE 27 FT /note="Transition state stabilizer" FT Condition: K FT SITE 157 FT /note="Positions MEP for the nucleophilic attack" FT Condition: R FT SITE 213 FT /note="Positions MEP for the nucleophilic attack" FT Condition: K XX Size: 211-291; Related: MF_01520!; Template: Q46893; Scope: Bacteria Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: See MF_01520 for bifunctional ispDF XX # Revision 1.33 2023/06/01 //