AC   MF_00159;
DC   Protein; auto
TR   HAMAP; MF_00159; -; 1; level=0
XX
Names: IspG
XX
ID   ISPG
case <OC:Cyanobacteriota>
DE   RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin);
DE            EC=1.17.7.1;
DE   AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase;
else
DE   RecName: Full=4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin);
DE            EC=1.17.7.3;
DE   AltName: Full=1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase;
end case
GN   Name=ispG;
XX
CC   -!- FUNCTION: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-
CC       2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.
case <OC:Cyanobacteriota> or <OG:Chloroplast>
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + H2O + 2
CC         oxidized [2Fe-2S]-[ferredoxin] = 2-C-methyl-D-erythritol 2,4-cyclic
CC         diphosphate + H(+) + 2 reduced [2Fe-2S]-[ferredoxin];
CC         Xref=Rhea:RHEA:26119, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737,
CC         ChEBI:CHEBI:33738, ChEBI:CHEBI:58483, ChEBI:CHEBI:128753;
CC         EC=1.17.7.1;
else
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O +
CC         oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic
CC         diphosphate + reduced [flavodoxin]; Xref=Rhea:RHEA:43604, Rhea:RHEA-
CC         COMP:10622, Rhea:RHEA-COMP:10623, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:58483, ChEBI:CHEBI:128753; EC=1.17.7.3;
end case
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 1 [4Fe-4S] cluster.;
end case
CC   -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis
CC       via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-
CC       phosphate: step 5/6.
CC   -!- SIMILARITY: Belongs to the IspG family.
XX
DR   Pfam; PF04551; GcpE; 1; trigger=no
DR   NCBIfam; TIGR00612; IspG_gcpE; 1; trigger=no
XX
case <FTGroup:1>
KW   4Fe-4S
KW   Metal-binding
KW   Iron
KW   Iron-sulfur
end case
KW   Isoprene biosynthesis
KW   Oxidoreductase
XX
case <FTGroup:1>
GO   GO:0051539; F:4 iron, 4 sulfur cluster binding
end case
GO   GO:0046429; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity
GO   GO:0016114; P:terpenoid biosynthetic process
XX
FT   From: ISPG_AQUAE (O67496)
FT   BINDING         265
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT   Group: 1; Condition: C
FT   BINDING         268
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT   Group: 1; Condition: C
FT   BINDING         300
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT   Group: 1; Condition: C
FT   BINDING         307
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT   Group: 1; Condition: E
XX
Size: 344-663;
Related: None;
Template: P62620; Q84GJ3; O67496; Q72H18;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: in STRAW, STRCO
Plasmid: None
Comments: Insertion in BACFN, BACFR, BACTN, CHLAB, CHLCV, CHLMU, CHLPN, CHLTE,
 CHLTR, LEPIC, LEPIN, PARUW and PORGI.
 Possible wrong start in CHLTE, DESVH and RHOPA.
XX
# Revision 1.38 2023/06/01
//