AC   MF_00171;
DC   Protein; auto
TR   HAMAP; MF_00171; -; 1; level=0
XX
Names: TruA
XX
ID   TRUA
DE   RecName: Full=tRNA pseudouridine synthase A;
DE            EC=5.4.99.12;
DE   AltName: Full=tRNA pseudouridine(38-40) synthase;
DE   AltName: Full=tRNA pseudouridylate synthase I;
DE   AltName: Full=tRNA-uridine isomerase I;
GN   Name=truA;
XX
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in the
CC       anticodon stem and loop of transfer RNAs.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC         Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
case <OC:Bacteria>
CC   -!- SUBUNIT: Homodimer.
end case
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
XX
DR   Pfam; PF01416; PseudoU_synth_1; 1; trigger=no
DR   NCBIfam; TIGR00071; HisT_truA; 1; trigger=no
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1; trigger=no
XX
KW   Isomerase
KW   tRNA processing
XX
GO   GO:0009982; F:pseudouridine synthase activity
GO   GO:0031119; P:tRNA pseudouridine synthesis
XX
FT   From: TRUA_ECOLI (P07649)
FT   REGION          107..111
FT                   /note="RNA binding"
FT   Optional; Group: 1; Condition: F-H-A-R-F
FT   REGION          168..172
FT                   /note="Interaction with tRNA"
FT   Optional; Group: 1; Condition: Q-C-Q-S-R
FT   ACT_SITE        60
FT                   /note="Nucleophile"
FT   Condition: D
FT   BINDING         118
FT                   /ligand="substrate"
FT   Condition: Y
FT   SITE            58
FT                   /note="Interaction with tRNA. Important for base-flipping"
FT   Optional; Group: 1; Condition: R
FT   SITE            78
FT                   /note="Interaction with tRNA"
FT   Optional; Group: 1; Condition: R
FT   SITE            110
FT                   /note="Interaction with tRNA"
FT   Optional; Group: 1; Condition: R
FT   SITE            126
FT                   /note="Interaction with tRNA"
FT   Optional; Group: 1; Condition: R
FT   SITE            139
FT                   /note="Interaction with tRNA"
FT   Optional; Group: 1; Condition: F
XX
Size: 240-304;
Related: None;
Template: P07649;
Scope:
 Bacteria
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: in BACAN, BACC1, BACCR, BACCZ, BACHK, CLOPE, CLOTE, DESPS, PARUW
Plasmid: None
Comments: Possible wrong start in MYCLE, MYCTU and MYCPA.
 Divergent LEPIN and NANEQ; not shown in alignment and not used in size range.
XX
# Revision 1.33 2023/06/01
//