AC   MF_00172;
DC   Protein; auto
TR   HAMAP; MF_00172; -; 1; level=0
XX
Names: Meth_synth
XX
ID   METE
DE   RecName: Full=5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase;
DE            EC=2.1.1.14;
DE   AltName: Full=Cobalamin-independent methionine synthase;
DE   AltName: Full=Methionine synthase, vitamin-B12 independent isozyme;
GN   Name=metE;
XX
CC   -!- FUNCTION: Catalyzes the transfer of a methyl group from 5-
CC       methyltetrahydrofolate to homocysteine resulting in methionine
CC       formation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-
CC         methionine + tetrahydropteroyltri-L-glutamate; Xref=Rhea:RHEA:21196,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:58140, ChEBI:CHEBI:58199,
CC         ChEBI:CHEBI:58207; EC=2.1.1.14;
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC       Note=Binds 1 zinc ion per subunit.;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC       pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
CC   -!- SIMILARITY: Belongs to the vitamin-B12 independent methionine synthase
CC       family.
XX
DR   Pfam; PF08267; Meth_synt_1; 1; trigger=no
DR   Pfam; PF01717; Meth_synt_2; 1; trigger=no
DR   PIRSF; PIRSF000382; MeTrfase_B12_ind; 1; trigger=no
DR   NCBIfam; TIGR01371; Met_syn_B12ind; 1; trigger=no
XX
KW   Amino-acid biosynthesis
KW   Metal-binding
KW   Methionine biosynthesis
KW   Methyltransferase
KW   Repeat
KW   Transferase
KW   Zinc
XX
GO   GO:0003871; F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity
GO   GO:0009086; P:methionine biosynthetic process
XX
FT   From: METE_THEMA (Q9X112)
FT   BINDING         15..18
FT                   /ligand="5-methyltetrahydropteroyltri-L-glutamate"
FT                   /ligand_id="ChEBI:CHEBI:58207"
FT   Optional; Condition: R-E-x-K
FT   BINDING         409..411
FT                   /ligand="L-homocysteine"
FT                   /ligand_id="ChEBI:CHEBI:58199"
FT   Optional; Condition: I-G-S
FT   BINDING         409..411
FT                   /ligand="L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:57844"
FT   Optional; Condition: I-G-S
FT   BINDING         493..494
FT                   /ligand="5-methyltetrahydropteroyltri-L-glutamate"
FT                   /ligand_id="ChEBI:CHEBI:58207"
FT   Optional; Condition: R-C
FT   ACT_SITE        672
FT                   /note="Proton donor"
FT   Condition: H
FT   BINDING         618
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT   Condition: H
FT   BINDING         620
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT   Condition: C
FT   BINDING         642
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT   Condition: E
FT   BINDING         704
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT   Condition: C
FT   BINDING         104
FT                   /ligand="5-methyltetrahydropteroyltri-L-glutamate"
FT                   /ligand_id="ChEBI:CHEBI:58207"
FT   Optional; Condition: [KR]
FT   BINDING         462
FT                   /ligand="L-homocysteine"
FT                   /ligand_id="ChEBI:CHEBI:58199"
FT   Optional; Condition: E
FT   BINDING         462
FT                   /ligand="L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:57844"
FT   Optional; Condition: E
FT   BINDING         539
FT                   /ligand="5-methyltetrahydropteroyltri-L-glutamate"
FT                   /ligand_id="ChEBI:CHEBI:58207"
FT   Optional; Condition: W
FT   BINDING         577
FT                   /ligand="L-homocysteine"
FT                   /ligand_id="ChEBI:CHEBI:58199"
FT   Optional; Condition: D
FT   BINDING         577
FT                   /ligand="L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:57844"
FT   Optional; Condition: D
FT   BINDING         583
FT                   /ligand="5-methyltetrahydropteroyltri-L-glutamate"
FT                   /ligand_id="ChEBI:CHEBI:58207"
FT   Optional; Condition: E
XX
Size: 734-792;
Related: MF_00288;
Template: P25665; Q9X112; Q8CWX6;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: in RALN1
Comments: None
XX
# Revision 1.35 2023/11/28
//