AC   MF_00185;
DC   Protein; auto
TR   HAMAP; MF_00185; -; 1; level=0
XX
Names: IPP_trans
XX
ID   MIAA
DE   RecName: Full=tRNA dimethylallyltransferase;
DE            EC=2.5.1.75;
DE   AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase;
DE            Short=DMAPP:tRNA dimethylallyltransferase;
DE            Short=DMATase;
DE   AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase;
DE            Short=IPP transferase;
DE            Short=IPPT;
DE            Short=IPTase;
GN   Name=miaA;
XX
CC   -!- FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the
CC       adenine at position 37 in tRNAs that read codons beginning with
CC       uridine, leading to the formation of N6-(dimethylallyl)adenosine
CC       (i(6)A).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(37) in tRNA + dimethylallyl diphosphate =
CC         diphosphate + N(6)-dimethylallyladenosine(37) in tRNA;
CC         Xref=Rhea:RHEA:26482, Rhea:RHEA-COMP:10162, Rhea:RHEA-COMP:10375,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:74411,
CC         ChEBI:CHEBI:74415; EC=2.5.1.75;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- SUBUNIT: Monomer.
CC   -!- SIMILARITY: Belongs to the IPP transferase family.
XX
DR   Pfam; PF01715; IPPT; 1; trigger=no
DR   NCBIfam; TIGR00174; MiaA; 1; trigger=no
XX
KW   ATP-binding
KW   Magnesium
KW   Nucleotide-binding
KW   Transferase
KW   tRNA processing
XX
GO   GO:0005524; F:ATP binding
GO   GO:0052381; F:tRNA dimethylallyltransferase activity
GO   GO:0008033; P:tRNA processing
XX
FT   From: MIAA_ECOLI (P16384)
FT   BINDING         17..24
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   BINDING         19..24
FT                   /ligand="substrate"
FT   Optional; Condition: [ST]-x-x-[GN]-K-[ST]
FT   REGION          42..45
FT                   /note="Interaction with substrate tRNA"
FT   Optional; Condition: D-S-x-x
FT   REGION          166..170
FT                   /note="Interaction with substrate tRNA"
FT   Optional; Condition: Q-R-x-x-R
FT   REGION          247..252
FT                   /note="Interaction with substrate tRNA"
FT   Optional; Condition: R-C-V-G-Y-R
FT   REGION          280..287
FT                   /note="Interaction with substrate tRNA"
FT   Optional; Condition: K-R-Q-I-T-W-L-R
FT   SITE            108
FT                   /note="Interaction with substrate tRNA"
FT   Optional; Condition: [TS]
FT   SITE            130
FT                   /note="Interaction with substrate tRNA"
FT   Optional; Condition: R
XX
Size: 279-363;
Related: None;
Template: P16384;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: in AZOPC, BACFN, BACFR, BACTN, PHOV8, CITBB, TRIL1, GEODF, GEOUR,
 HAHCH, MYCMM, MYCUA, PARD8, SYNC1, PELPD, PORG3, PORGI, SHESH, SYNAS, TREDE
Plasmid: in THERP
Comments: Possible wrong start in a number of cases
XX
# Revision 1.34 2023/06/01
//