AC MF_00193; DC Protein; auto TR HAMAP; MF_00193; -; 1; level=0 XX Names: NadE_ammonia_dep XX ID NADE DE RecName: Full=NH(3)-dependent NAD(+) synthetase; DE EC=6.3.1.5; GN Name=nadE; XX CC -!- FUNCTION: Catalyzes the ATP-dependent amidation of deamido-NAD to form CC NAD. Uses ammonia as a nitrogen source. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + CC NAD(+); Xref=Rhea:RHEA:21188, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.1.5; CC -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from CC deamido-NAD(+) (ammonia route): step 1/1. CC -!- SUBUNIT: Homodimer. CC -!- SIMILARITY: Belongs to the NAD synthetase family. XX DR Pfam; PF02540; NAD_synthase; 1; trigger=no DR NCBIfam; TIGR00552; nadE; 1; trigger=no XX KW ATP-binding KW Ligase KW Magnesium KW Metal-binding KW NAD KW Nucleotide-binding XX GO GO:0005524; F:ATP binding GO GO:0008795; F:NAD+ synthase activity GO GO:0009435; P:NAD biosynthetic process XX FT From: NADE_BACSU (P08164) FT BINDING 45..52 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: [GA]-[ILVM]-S-G-G-x-D-S FT BINDING 258..259 FT /ligand="deamido-NAD(+)" FT /ligand_id="ChEBI:CHEBI:58437" FT /ligand_note="ligand shared between two neighboring FT subunits" FT /note="in other chain" FT Optional; Condition: H-[KR] FT BINDING 51 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT Condition: D FT BINDING 163 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT Condition: E FT BINDING 138 FT /ligand="deamido-NAD(+)" FT /ligand_id="ChEBI:CHEBI:58437" FT /ligand_note="ligand shared between two neighboring FT subunits" FT /note="in other chain" FT Optional; Condition: R FT BINDING 158 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Optional; Condition: T FT BINDING 171 FT /ligand="deamido-NAD(+)" FT /ligand_id="ChEBI:CHEBI:58437" FT /ligand_note="ligand shared between two neighboring FT subunits" FT /note="in other chain" FT Optional; Condition: K FT BINDING 178 FT /ligand="deamido-NAD(+)" FT /ligand_id="ChEBI:CHEBI:58437" FT /ligand_note="ligand shared between two neighboring FT subunits" FT Optional; Condition: D FT BINDING 187 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Optional; Condition: K FT BINDING 209 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Optional; Condition: [ST] XX Size: 230-350; Related: None; Template: P08164; P18843; Q81RP3; Scope: Bacteria Archaea Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.37 2023/06/01 //