AC   MF_00281;
DC   Protein; auto
TR   HAMAP; MF_00281; -; 1; level=0
XX
Names: Phe_tRNA_synth_alpha1
XX
ID   SYFA
DE   RecName: Full=Phenylalanine--tRNA ligase alpha subunit;
DE            EC=6.1.1.20;
DE   AltName: Full=Phenylalanyl-tRNA synthetase alpha subunit;
DE            Short=PheRS;
GN   Name=pheS;
XX
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) +
CC         AMP + diphosphate + H(+); Xref=Rhea:RHEA:19413, Rhea:RHEA-COMP:9668,
CC         Rhea:RHEA-COMP:9699, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58095, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78531, ChEBI:CHEBI:456215; EC=6.1.1.20;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Note=Binds 2 magnesium ions per tetramer.;
CC   -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       Phe-tRNA synthetase alpha subunit type 1 subfamily.
XX
DR   Pfam; PF01409; tRNA-synt_2d; 1; trigger=no
DR   NCBIfam; TIGR00468; PheS; 1; trigger=no
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1; trigger=no
XX
KW   Cytoplasm
KW   Aminoacyl-tRNA synthetase
KW   Protein biosynthesis
KW   Ligase
KW   ATP-binding
KW   Nucleotide-binding
KW   Magnesium
KW   Metal-binding
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GO   GO:0005524; F:ATP binding
GO   GO:0000287; F:magnesium ion binding
GO   GO:0004826; F:phenylalanine-tRNA ligase activity
GO   GO:0006432; P:phenylalanyl-tRNA aminoacylation
GO   GO:0005737; C:cytoplasm
XX
FT   From: SYFA_THETH (P27001)
FT   BINDING         262
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="shared with beta subunit"
FT   Condition: E
XX
Size: 325-373;
Related: MF_00282;
Template: P08312; P27001;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: BUCAI and BUCAP lack the conserved Glu that bind magnesium
XX
# Version: 24
# Last updated date: 2024-09-02
# Created date: 2005-02-28
//