AC   MF_00296;
DC   Protein; auto
TR   HAMAP; MF_00296; -; 1; level=0
XX
Names: MetX_acyltransf
XX
case <FTGroup:1> and not (<FTTag:P11> or <FTTag:P17>)
ID   METXA
DE   RecName: Full=Homoserine O-acetyltransferase;
DE            Short=HAT;
DE            EC=2.3.1.31;
DE   AltName: Full=Homoserine transacetylase;
DE            Short=HTA;
GN   Name=metXA;
else case <FTGroup:1> and <FTTag:P17>
ID   METXS
DE   RecName: Full=Homoserine O-succinyltransferase;
DE            Short=HST;
DE            EC=2.3.1.46;
DE   AltName: Full=Homoserine transsuccinylase;
DE            Short=HTS;
GN   Name=metXS;
else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and <FTTag:P9>
ID   METXS
DE   RecName: Full=Homoserine O-succinyltransferase;
DE            Short=HST;
DE            EC=2.3.1.46;
DE   AltName: Full=Homoserine transsuccinylase;
DE            Short=HTS;
GN   Name=metXS;
else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and not <FTTag:P9>
ID   SST
DE   RecName: Full=Serine O-succinyltransferase;
DE            Short=SST;
DE            EC=2.3.1.-;
else
ID   METX
DE   RecName: Full=Probable acyltransferase;
DE            EC=2.3.1.-;
end case
XX
case <FTGroup:1> and not (<FTTag:P11> or <FTTag:P17>)
CC   -!- FUNCTION: Transfers an acetyl group from acetyl-CoA to L-homoserine,
CC       forming acetyl-L-homoserine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine;
CC         Xref=Rhea:RHEA:13701, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:57476, ChEBI:CHEBI:57716; EC=2.3.1.31;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC       pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.
else case (<FTGroup:1> and <FTTag:P17>) or (<FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and <FTTag:P9>)
CC   -!- FUNCTION: Transfers a succinyl group from succinyl-CoA to L-homoserine,
CC       forming succinyl-L-homoserine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-homoserine + succinyl-CoA = CoA + O-succinyl-L-homoserine;
CC         Xref=Rhea:RHEA:22008, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292,
CC         ChEBI:CHEBI:57476, ChEBI:CHEBI:57661; EC=2.3.1.46;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC       pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1.
else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and not <FTTag:P9>
CC   -!- FUNCTION: Transfers a succinyl group from succinyl-CoA to L-serine,
CC       forming succinyl-L-serine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-serine + succinyl-CoA = CoA + O-succinyl-L-serine;
CC         Xref=Rhea:RHEA:52820, ChEBI:CHEBI:33384, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57292, ChEBI:CHEBI:136856;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine
CC       from L-serine: step 1/2.
end case
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. MetX family.
XX
DR   Pfam; PF00561; Abhydrolase_1; 1; trigger=yes
DR   PIRSF; PIRSF000443; Homoser_Ac_trans; 1; trigger=no
DR   NCBIfam; TIGR01392; homoserO_Ac_trn; 1; trigger=no
DR   PROSITE; PS51371; CBS; 0-2; trigger=yes
XX
KW   Cytoplasm
KW   Amino-acid biosynthesis
case <FTGroup:1> and not (<FTTag:P11> or <FTTag:P17>)
KW   Methionine biosynthesis
else case (<FTGroup:1> and <FTTag:P17>) or (<FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and <FTTag:P9>)
KW   Methionine biosynthesis
else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and not <FTTag:P9>
KW   Cysteine biosynthesis
end case
KW   Transferase
KW   Acyltransferase
XX
case <FTGroup:1> and not (<FTTag:P11> or <FTTag:P17>)
GO   GO:0004414; F:homoserine O-acetyltransferase activity
GO   GO:0009086; P:methionine biosynthetic process
else case (<FTGroup:1> and <FTTag:P17>) or (<FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and <FTTag:P9>)
GO   GO:0008899; F:homoserine O-succinyltransferase activity
GO   GO:0009086; P:methionine biosynthetic process
else case <FTGroup:1> and <FTTag:P11> and <FTTag:P1P4> and not <FTTag:P9>
GO   GO:0016750; F:O-succinyltransferase activity
GO   GO:0006535; P:cysteine biosynthetic process from serine
end case
GO   GO:0005737; C:cytoplasm
XX
FT   From: METXA_HAEIN (P45131)
FT   REGION          48..51
FT                   /note="Important for substrate specificity"
FT   Tag: P1P4; Optional; Condition: G-[LI]-S-[PA]
FT   ACT_SITE        143
FT                   /note="Nucleophile"
FT   Group: 1; Condition: S
FT   ACT_SITE        304
FT   Condition: D
FT   ACT_SITE        337
FT   Group: 1; Condition: H
FT   BINDING         212
FT                   /ligand="substrate"
FT   Group: 1; Condition: R
FT   BINDING         338
FT                   /ligand="substrate"
FT   Group: 1; Condition: D
FT   SITE            144
FT                   /note="Important for substrate specificity"
FT   Tag: P9; Optional; Condition: Y
FT   SITE            180
FT                   /note="Important for acyl-CoA specificity"
FT   Tag: P11; Optional; Condition: R
FT   SITE            306
FT                   /note="Important for acyl-CoA specificity"
FT   Tag: P17; Optional; Condition: R
XX
Size: 315-545;
Related: None;
Template: P45131; Q8F4I0; O68640; Q9RA51; Q6FEQ3; P57714; Q8P8L2; Q8TME4;
Scope:
 Bacteria
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: Classification as HST, HAT or SST was done according to Bastard et al. (2017) (PubMed:28581482).
XX
# Revision 1.42 2023/06/01
//