AC   MF_00379;
DC   Protein; auto
c?   <OC:Bacteria> or <OG:Chloroplast>
TR   HAMAP; MF_00379; -; 1; level=0
XX
Names: GTPase_MnmE
XX
ID   MNME
case <OC:Bacteria>
DE   RecName: Full=tRNA modification GTPase MnmE;
DE            EC=3.6.-.-;
GN   Name=mnmE; Synonyms=trmE;
else case <OG:Chloroplast>
DE   RecName: Full=Probable tRNA modification GTPase MnmE;
DE            EC=3.6.-.-;
GN   Name=mnmE; Synonyms=thdF;
end case
XX
CC   -!- FUNCTION: Exhibits a very high intrinsic GTPase hydrolysis rate.
CC       Involved in the addition of a carboxymethylaminomethyl (cmnm) group at
CC       the wobble position (U34) of certain tRNAs, forming tRNA-
CC       cmnm(5)s(2)U34.
CC   -!- COFACTOR:
CC       Name=K(+); Xref=ChEBI:CHEBI:29103;
CC       Note=Binds 1 potassium ion per subunit.;
case <OC:Bacteria>
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
else case <OG:Chloroplast>
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
end case
CC   -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC       GTPase superfamily. TrmE GTPase family.
XX
DR   Pfam; PF01926; MMR_HSR1; 1; trigger=no
DR   Pfam; PF10396; TrmE_N; 1; trigger=no
DR   PRINTS; PR00326; GTP1OBG; 1; trigger=no
DR   NCBIfam; TIGR00450; MnmE_trmE_thdF; 1; trigger=no
DR   NCBIfam; TIGR00231; Small_GTP; 1; trigger=no
DR   PROSITE; PS51709; G_TRME; 1; trigger=no
XX
case <OC:Bacteria>
KW   Cytoplasm
end case
KW   GTP-binding
KW   Hydrolase
KW   Magnesium
KW   Metal-binding
KW   Nucleotide-binding
KW   Potassium
KW   tRNA processing
XX
GO   GO:0005525; F:GTP binding
GO   GO:0003924; F:GTPase activity
GO   GO:0006400; P:tRNA modification
case <OC:Bacteria>
GO   GO:0005737; C:cytoplasm
else case <OG:Chloroplast>
GO   GO:0009507; C:chloroplast
end case
XX
FT   From: MNME_ECOLI (P25522)
FT   BINDING         226..231
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: N-[VSAT]-G-K-S-[TSQR]
FT   BINDING         245..251
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: [TSMAD]-x(3)-G-[TSR]-T
FT   BINDING         270..273
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: D-[TS]-A-G
FT   BINDING         335..338
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Optional; Condition: N-K-A-D
FT   BINDING         358..360
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Optional; Condition: S-A-R
FT   BINDING         226
FT                   /ligand="K(+)"
FT                   /ligand_id="ChEBI:CHEBI:29103"
FT   Optional; Group: 1; Condition: N
FT   BINDING         230
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: S
FT   BINDING         245
FT                   /ligand="K(+)"
FT                   /ligand_id="ChEBI:CHEBI:29103"
FT   Optional; Group: 1; Condition: [TS]
FT   BINDING         247
FT                   /ligand="K(+)"
FT                   /ligand_id="ChEBI:CHEBI:29103"
FT   Optional; Group: 1; Condition: [IVL]
FT   BINDING         250
FT                   /ligand="K(+)"
FT                   /ligand_id="ChEBI:CHEBI:29103"
FT   Optional; Group: 1; Condition: [TS]
FT   BINDING         251
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: T
FT   BINDING         23
FT                   /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57457"
FT   Group: 2; Condition: [RK]
FT   BINDING         80
FT                   /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57457"
FT   Group: 2; Condition: E
FT   BINDING         120
FT                   /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57457"
FT   Group: 2; Condition: [RKVM]
FT   BINDING         454
FT                   /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate"
FT                   /ligand_id="ChEBI:CHEBI:57457"
FT   Group: 2; Condition: K
XX
Size: 419-550;
Related: None;
Template: P25522; Q9WYA4;
Scope:
 Bacteria
 Plastid
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.36 2023/06/01
//