AC   MF_00394;
DC   Protein; auto
TR   HAMAP; MF_00394; -; 1; level=0
XX
Names: NAD_Glyc3P_dehydrog
XX
ID   GPDA
DE   RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD(P)+];
DE            EC=1.1.1.94;
DE   AltName: Full=NAD(P)(+)-dependent glycerol-3-phosphate dehydrogenase;
DE   AltName: Full=NAD(P)H-dependent dihydroxyacetone-phosphate reductase;
GN   Name=gpsA;
XX
case <OC:Bacteria>
CC   -!- FUNCTION: Catalyzes the reduction of the glycolytic intermediate
CC       dihydroxyacetone phosphate (DHAP) to sn-glycerol 3-phosphate (G3P), the
CC       key precursor for phospholipid synthesis.
else case <OC:Archaea>
CC   -!- FUNCTION: Catalyzes the reduction of the glycolytic intermediate
CC       dihydroxyacetone phosphate (DHAP) to sn-glycerol 3-phosphate (G3P).
end case
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate
CC         + NADH + H(+); Xref=Rhea:RHEA:11092, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57597, ChEBI:CHEBI:57642,
CC         ChEBI:CHEBI:57945; EC=1.1.1.94;
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11094;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=sn-glycerol 3-phosphate + NADP(+) = dihydroxyacetone phosphate
CC         + NADPH + H(+); Xref=Rhea:RHEA:11096, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57597, ChEBI:CHEBI:57642, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.1.1.94;
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11098;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
case <OC:Bacteria>
CC   -!- PATHWAY: Membrane lipid metabolism; glycerophospholipid metabolism.
end case
CC   -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC       dehydrogenase family.
XX
DR   Pfam; PF07479; NAD_Gly3P_dh_C; 1; trigger=no
DR   Pfam; PF01210; NAD_Gly3P_dh_N; 1; trigger=no
DR   PIRSF; PIRSF000114; Glycerol-3-P_dh; 1; trigger=no
DR   PRINTS; PR00077; GPDHDRGNASE; 1; trigger=no
DR   PROSITE; PS00957; NAD_G3PDH; 1; trigger=no
XX
KW   Cytoplasm
case <OC:Bacteria>
KW   Lipid biosynthesis
KW   Lipid metabolism
KW   Phospholipid biosynthesis
KW   Phospholipid metabolism
end case
KW   NAD
KW   NADP
KW   Oxidoreductase
KW   Nucleotide-binding
XX
GO   GO:0047952; F:glycerol-3-phosphate dehydrogenase [NAD(P)+] activity
GO   GO:0046167; P:glycerol-3-phosphate biosynthetic process
GO   GO:0006650; P:glycerophospholipid metabolic process
GO   GO:0005737; C:cytoplasm
XX
FT   From: GPDA_THESQ (A0A0F6AK91)
FT   ACT_SITE        177
FT                   /note="Proton acceptor"
FT   Condition: K
FT   BINDING         10
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Optional; Condition: [ST]
FT   BINDING         11
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Optional; Condition: [WYF]
FT   BINDING         31
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Optional; Condition: [RHK]
FT   BINDING         32
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Optional; Condition: [RHK]
FT   BINDING         47
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Optional; Condition: Y
FT   BINDING         98
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Condition: K
FT   BINDING         98
FT                   /ligand="sn-glycerol 3-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57597"
FT   Condition: K
FT   BINDING         125
FT                   /ligand="sn-glycerol 3-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57597"
FT   Condition: G
FT   BINDING         127
FT                   /ligand="sn-glycerol 3-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57597"
FT   Optional; Condition: [ST]
FT   BINDING         129
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Condition: [AGS]
FT   BINDING         177
FT                   /ligand="sn-glycerol 3-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57597"
FT   Condition: K
FT   BINDING         230
FT                   /ligand="sn-glycerol 3-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57597"
FT   Condition: D
FT   BINDING         240
FT                   /ligand="sn-glycerol 3-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57597"
FT   Optional; Condition: S
FT   BINDING         241
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Condition: R
FT   BINDING         241
FT                   /ligand="sn-glycerol 3-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57597"
FT   Condition: R
FT   BINDING         242
FT                   /ligand="sn-glycerol 3-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:57597"
FT   Condition: N
FT   BINDING         265
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Optional; Condition: [VLI]
FT   BINDING         267
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Condition: E
XX
Size: 297-369;
Related: None;
Template: P0A6S7; A0A0F6AK91; P46919; O29390;
Scope:
 Bacteria
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: in MYCPA, MYCTU
Plasmid: None
Comments: None
XX
# Version: 35
# Last updated date: 2024-09-02
# Created date: 2005-02-28
//