AC   MF_00419;
DC   Protein; auto
TR   HAMAP; MF_00419; -; 1; level=0
XX
Names: PurL_1
XX
ID   PUR4
DE   RecName: Full=Phosphoribosylformylglycinamidine synthase;
DE            Short=FGAM synthase;
DE            Short=FGAMS;
DE            EC=6.3.5.3;
DE   AltName: Full=Formylglycinamide ribonucleotide amidotransferase;
DE            Short=FGAR amidotransferase;
DE            Short=FGAR-AT;
GN   Name=purL;
XX
CC   -!- FUNCTION: Phosphoribosylformylglycinamidine synthase involved in the
CC       purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of
CC       formylglycinamide ribonucleotide (FGAR) and glutamine to yield
CC       formylglycinamidine ribonucleotide (FGAM) and glutamate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-
CC         ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-
CC         ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate;
CC         Xref=Rhea:RHEA:17129, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:58359, ChEBI:CHEBI:147286, ChEBI:CHEBI:147287,
CC         ChEBI:CHEBI:456216; EC=6.3.5.3;
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC       amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-
CC       phospho-D-ribosyl)glycinamide: step 1/2.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the FGAMS family.
XX
DR   Pfam; PF00586; AIRS; 1; trigger=no
DR   PROSITE; PS51273; GATASE_TYPE_1; 1; trigger=yes
DR   NCBIfam; TIGR01735; FGAM_synt; 1; trigger=no
XX
KW   ATP-binding
KW   Cytoplasm
KW   Glutamine amidotransferase
KW   Ligase
KW   Metal-binding
KW   Magnesium
KW   Nucleotide-binding
KW   Purine biosynthesis
XX
GO   GO:0005524; F:ATP binding
GO   GO:0004642; F:phosphoribosylformylglycinamidine synthase activity
GO   GO:0006189; P:'de novo' IMP biosynthetic process
GO   GO:0005737; C:cytoplasm
XX
FT   From: PUR4_SALTY (P74881)
FT   BINDING         307..318
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Optional; Condition: G-A-[SA]-T-G-[SAVI]-G-G-E-I-R-D
FT   BINDING         386..388
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Optional; Condition: T-G-Y
FT   ACT_SITE        1135
FT                   /note="Nucleophile"
FT   Condition: C
FT   ACT_SITE        1260
FT   Condition: H
FT   ACT_SITE        1262
FT   Condition: E
FT   BINDING         679
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: D
FT   BINDING         718
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: E
FT   BINDING         722
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: N
FT   BINDING         884
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: D
FT   BINDING         678
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: A
FT   BINDING         886
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Condition: S
XX
Size: 1293-1369;
Related: MF_00420; MF_00421;
Template: P15254; P74881;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419),
 or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421)
 and PurS (MF_01926).
XX
# Revision 1.47 2023/06/01
//