AC   MF_00518;
DC   Protein; auto
TR   HAMAP; MF_00518; -; 1; level=0
XX
Names: Deacylase_Dtd
XX
ID   DTD
DE   RecName: Full=D-aminoacyl-tRNA deacylase;
DE            Short=DTD;
DE            EC=3.1.1.96;
DE   AltName: Full=Gly-tRNA(Ala) deacylase;
DE            EC=3.1.1.-;
GN   Name=dtd;
XX
CC   -!- FUNCTION: An aminoacyl-tRNA editing enzyme that deacylates mischarged
CC       D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala),
CC       protecting cells against glycine mischarging by AlaRS. Acts via tRNA-
CC       based rather than protein-based catalysis; rejects L-amino acids rather
CC       than detecting D-amino acids in the active site. By recycling D-
CC       aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme
CC       counteracts the toxicity associated with the formation of D-aminoacyl-
CC       tRNA entities in vivo and helps enforce protein L-homochirality.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+);
CC         Xref=Rhea:RHEA:13953, Rhea:RHEA-COMP:10123, Rhea:RHEA-COMP:10124,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:59871,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:79333; EC=3.1.1.96;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala);
CC         Xref=Rhea:RHEA:53744, Rhea:RHEA-COMP:9657, Rhea:RHEA-COMP:13640,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78522;
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
case <FTTag:motif>
CC   -!- DOMAIN: A Gly-cisPro motif from one monomer fits into the active site
CC       of the other monomer to allow specific chiral rejection of L-amino
CC       acids.
end case
CC   -!- SIMILARITY: Belongs to the DTD family.
XX
DR   Pfam; PF02580; Tyr_Deacylase; 1; trigger=no
DR   NCBIfam; TIGR00256; TIGR00256; 1; trigger=no
XX
KW   Cytoplasm
KW   Hydrolase
KW   RNA-binding
KW   tRNA-binding
XX
GO   GO:0016788; F:hydrolase activity, acting on ester bonds
GO   GO:0019478; P:D-amino acid catabolic process
GO   GO:0051499; F:D-aminoacyl-tRNA deacylase activity
GO   GO:0005737; C:cytoplasm
GO   GO:0000049; F:tRNA binding
GO   GO:0043908; F:Ser(Gly)-tRNA(Ala) hydrolase activity
GO   GO:0106026; F:Gly-tRNA(Ala) hydrolase activity
XX
FT   From: DTD_ECOLI (P0A6M4)
FT   MOTIF           137..138
FT                   /note="Gly-cisPro motif, important for rejection of L-amino
FT                   acids"
FT   Tag: motif; Condition: G-P
XX
Size: 127-200;
Related: None;
Template: P0A6M4; Q8IIS0;
Scope:
 Bacteria
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: BACSU (O32042) is inactive due to missing start codon, SHIF8
 (Q0SZ73) may have a sequence error
XX
# Revision 1.31 2023/06/01
//