AC MF_00553; DC Protein; auto TR HAMAP; MF_00553; -; 1; level=0 XX Names: PAN XX ID PAN DE RecName: Full=Proteasome-activating nucleotidase; DE Short=PAN; DE AltName: Full=Proteasomal ATPase; DE AltName: Full=Proteasome regulatory ATPase; DE AltName: Full=Proteasome regulatory particle; GN Name=pan; XX CC -!- FUNCTION: ATPase which is responsible for recognizing, binding, CC unfolding and translocation of substrate proteins into the archaeal 20S CC proteasome core particle. Is essential for opening the gate of the 20S CC proteasome via an interaction with its C-terminus, thereby allowing CC substrate entry and access to the site of proteolysis. Thus, the C- CC termini of the proteasomal ATPase function like a 'key in a lock' to CC induce gate opening and therefore regulate proteolysis. Unfolding CC activity requires energy from ATP hydrolysis, whereas ATP binding alone CC promotes ATPase-20S proteasome association which triggers gate opening, CC and supports translocation of unfolded substrates. CC -!- SUBUNIT: Homohexamer. The hexameric complex has a two-ring architecture CC resembling a top hat that caps the 20S proteasome core at one or both CC ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha- CC rings of the proteasome core by binding to the intersubunit pockets. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- DOMAIN: Consists of three main regions, an N-terminal coiled-coil CC domain that may assist in substrate recognition, an interdomain CC involved in PAN hexamerization, and a C-terminal ATPase domain of the CC AAA type. CC -!- SIMILARITY: Belongs to the AAA ATPase family. XX DR PROSITE; PS00674; AAA; 1; trigger=no DR Pfam; PF00004; AAA; 1; trigger=no DR NCBIfam; TIGR01242; 26Sp45; 1; trigger=no DR General; Coiled_coil; -; 0-unlimited; trigger=yes XX KW Chaperone KW Cytoplasm KW Proteasome KW ATP-binding KW Nucleotide-binding XX GO GO:0005524; F:ATP binding GO GO:0016887; F:ATP hydrolysis activity GO GO:0010498; P:proteasomal protein catabolic process GO GO:0043335; P:protein unfolding GO GO:0000502; C:proteasome complex GO GO:0005737; C:cytoplasm GO GO:0022623; C:proteasome-activating nucleotidase complex XX FT From: PAN_METJA (Q58576) FT BINDING 214..219 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: G-x-G-K-T-[LM] FT REGION 428..430 FT /note="Docks into pockets in the proteasome alpha-ring to FT cause gate opening" FT Condition: x-[YF]-x FT BINDING 353 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: [HY] XX Size: 392-436; Related: None; Template: Q58576; O28303; D4GUJ7; Q5UT56; Scope: Archaea Fusion: Nter: None Cter: None Duplicate: in HALSA, HALVD Plasmid: None Comments: The protein performing the same function in bacteria (called ARC) is in MF_02112. XX # Revision 1.31 2023/06/01 //