AC   MF_00554;
DC   Protein; auto
TR   HAMAP; MF_00554; -; 1; level=0
XX
Names: NEP1
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ID   NEP1
DE   RecName: Full=Ribosomal RNA small subunit methyltransferase Nep1;
DE            EC=2.1.1.-;
DE   AltName: Full=16S rRNA (pseudouridine-N1-)-methyltransferase Nep1;
GN   Name=nep1;
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CC   -!- FUNCTION: Methyltransferase involved in ribosomal biogenesis.
CC       Specifically catalyzes the N1-methylation of the pseudouridine
CC       corresponding to position 914 in M.jannaschii 16S rRNA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a pseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = an
CC         N(1)-methylpseudouridine in 16S/18S rRNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:46696, Rhea:RHEA-COMP:11633, Rhea:RHEA-
CC         COMP:11634, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:74890;
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. RNA methyltransferase NEP1 family.
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DR   Pfam; PF03587; EMG1; 1; trigger=no
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KW   Methyltransferase
KW   Ribosome biogenesis
KW   RNA-binding
KW   rRNA-binding
KW   rRNA processing
KW   S-adenosyl-L-methionine
KW   Transferase
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GO   GO:0019843; F:rRNA binding
GO   GO:0070037; F:rRNA (pseudouridine) methyltransferase activity
GO   GO:0042254; P:ribosome biogenesis
GO   GO:0006364; P:rRNA processing
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FT   From: NEP1_METJA (Q57977)
FT   BINDING         185..190
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   BINDING         162
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: G
FT   BINDING         167
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: G
FT   SITE            54
FT                   /note="Interaction with substrate rRNA"
FT   Condition: R
FT   SITE            56
FT                   /note="Stabilizes Arg-xx"
FT   Condition: D
FT   SITE            95
FT                   /note="Interaction with substrate rRNA"
FT   Condition: R
FT   SITE            98
FT                   /note="Interaction with substrate rRNA"
FT   Condition: [RK]
FT   SITE            102
FT                   /note="Interaction with substrate rRNA"
FT   Condition: [RN]
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Size: 205-232;
Related: None;
Template: Q57977; Q06287;
Scope:
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
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# Revision 1.28 2022/11/19
//