AC MF_00588; DC Protein; auto TR HAMAP; MF_00588; -; 1; level=0 XX Names: GatE XX ID GATE DE RecName: Full=Glutamyl-tRNA(Gln) amidotransferase subunit E; DE Short=Glu-ADT subunit E; DE EC=6.3.5.-; GN Name=gatE; XX CC -!- FUNCTION: Allows the formation of correctly charged Gln-tRNA(Gln) CC through the transamidation of misacylated Glu-tRNA(Gln) in organisms CC which lack glutaminyl-tRNA synthetase. The reaction takes place in the CC presence of glutamine and ATP through an activated gamma-phospho-Glu- CC tRNA(Gln). The GatDE system is specific for glutamate and does not act CC on aspartate. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + CC L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate; CC Xref=Rhea:RHEA:17521, Rhea:RHEA-COMP:9681, Rhea:RHEA-COMP:9684, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, CC ChEBI:CHEBI:78520, ChEBI:CHEBI:78521, ChEBI:CHEBI:456216; CC -!- SUBUNIT: Heterodimer of GatD and GatE. CC -!- SIMILARITY: Belongs to the GatB/GatE family. GatE subfamily. XX DR Pfam; PF02938; GAD; 1; trigger=no DR Pfam; PF02934; GatB_N; 1; trigger=no DR Pfam; PF02637; GatB_Yqey; 1; trigger=no DR NCBIfam; TIGR00134; GatE_arch; 1; trigger=no DR PROSITE; PS01234; GATB; 1; trigger=no XX KW ATP-binding KW Ligase KW Nucleotide-binding KW Protein biosynthesis XX GO GO:0050567; F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity GO GO:0006412; P:translation XX FT None XX Size: 598-642; Related: MF_00121; Template: O26803; Scope: Archaea Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.21 2023/06/01 //