AC   MF_00614;
DC   Protein; auto
c?   <OC:Archaea>
TR   HAMAP; MF_00614; -; 1; level=0
XX
Names: Fen
XX
ID   FEN
DE   RecName: Full=Flap endonuclease 1;
DE            Short=FEN-1;
DE            EC=3.1.-.-;
DE   AltName: Full=Flap structure-specific endonuclease 1;
GN   Name=fen;
XX
CC   -!- FUNCTION: Structure-specific nuclease with 5'-flap endonuclease and 5'-
CC       3' exonuclease activities involved in DNA replication and repair.
CC       During DNA replication, cleaves the 5'-overhanging flap structure that
CC       is generated by displacement synthesis when DNA polymerase encounters
CC       the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA
CC       end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves
CC       one nucleotide into the double-stranded DNA from the junction in flap
CC       DNA, leaving a nick for ligation. Also involved in the base excision
CC       repair (BER) pathway. Acts as a genome stabilization factor that
CC       prevents flaps from equilibrating into structurs that lead to
CC       duplications and deletions. Also possesses 5'-3' exonuclease activity
CC       on nicked or gapped double-stranded DNA.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Note=Binds 2 magnesium ions per subunit. They probably participate in
CC       the reaction catalyzed by the enzyme. May bind an additional third
CC       magnesium ion after substrate binding.;
CC   -!- SUBUNIT: Interacts with PCNA. PCNA stimulates the nuclease activity
CC       without altering cleavage specificity.
CC   -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. FEN1
CC       subfamily.
XX
DR   Pfam; PF00867; XPG_I; 1; trigger=no
DR   Pfam; PF00752; XPG_N; 1; trigger=no
DR   PRINTS; PR00853; XPGRADSUPER; 1; trigger=no
DR   NCBIfam; TIGR03674; Fen_arch; 1; trigger=no
DR   PROSITE; PS00841; XPG_1; 1; trigger=no
XX
KW   DNA damage
KW   DNA repair
KW   DNA replication
KW   Endonuclease
KW   Exonuclease
KW   Hydrolase
KW   Magnesium
KW   Metal-binding
KW   Nuclease
XX
GO   GO:0003677; F:DNA binding
GO   GO:0008409; F:5'-3' exonuclease activity
GO   GO:0017108; F:5'-flap endonuclease activity
GO   GO:0000287; F:magnesium ion binding
GO   GO:0043137; P:DNA replication, removal of RNA primer
GO   GO:0006281; P:DNA repair
XX
FT   From: FEN_METJA (Q58839)
FT   REGION          1..98
FT                   /note="N-domain"
FT   REGION          116..245
FT                   /note="I-domain"
FT   REGION          317..325
FT                   /note="Interaction with PCNA"
FT   Optional; Condition: x-Q-x(2)-[ILM]-x(2)-[FYW]-[FYILAT]
FT   BINDING         27
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT   Condition: D
FT   BINDING         80
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT   Condition: D
FT   BINDING         152
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT   Condition: E
FT   BINDING         154
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT   Condition: E
FT   BINDING         173
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT   Condition: D
FT   BINDING         175
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT   Condition: D
FT   BINDING         224
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT   Condition: D
XX
Size: 326-351;
Related: None;
Template: Q58839; O93634;
Scope:
 Archaea
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.23 2023/06/01
//