AC MF_00639; DC Protein; auto TR HAMAP; MF_00639; -; 1; level=0 XX Names: MurD XX ID MURD DE RecName: Full=UDP-N-acetylmuramoylalanine--D-glutamate ligase; DE EC=6.3.2.9; DE AltName: Full=D-glutamic acid-adding enzyme; DE AltName: Full=UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase; GN Name=murD; XX CC -!- FUNCTION: Cell wall formation. Catalyzes the addition of glutamate to CC the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + D-glutamate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine = CC ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D- CC glutamate; Xref=Rhea:RHEA:16429, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:29986, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, CC ChEBI:CHEBI:83898, ChEBI:CHEBI:83900, ChEBI:CHEBI:456216; EC=6.3.2.9; CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: Belongs to the MurCDEF family. XX DR Pfam; PF01225; Mur_ligase; 1; trigger=no DR Pfam; PF02875; Mur_ligase_C; 1; trigger=no DR NCBIfam; TIGR01087; MurD; 1; trigger=no XX KW ATP-binding KW Cell cycle KW Cell division KW Cell shape KW Cell wall biogenesis/degradation KW Cytoplasm KW Ligase KW Nucleotide-binding KW Peptidoglycan synthesis XX GO GO:0005524; F:ATP binding GO GO:0008764; F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity GO GO:0009252; P:peptidoglycan biosynthetic process GO GO:0005737; C:cytoplasm XX FT From: MURD_ECOLI (P14900) FT BINDING 112..118 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: G-[STA]-x-[GA]-K-[GST]-[ST] XX Size: 380-660; Related: MF_02208!; Template: P14900; Q9HVZ9; Scope: Bacteria Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: Also matches MurD2 subfamily (MF_02208). Insertion in POLSJ (not shown in alignment and not used in size range) XX # Revision 1.35 2023/06/01 //