AC   MF_00641;
DC   Protein; auto
TR   HAMAP; MF_00641; -; 1; level=0
XX
Names: Malate_synth_G
XX
ID   MASZ
DE   RecName: Full=Malate synthase G;
DE            EC=2.3.3.9;
GN   Name=glcB;
XX
CC   -!- FUNCTION: Involved in the glycolate utilization. Catalyzes the
CC       condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-
CC       CoA) and glyoxylate to form malate and CoA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC         Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.9;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC       isocitrate: step 2/2.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the malate synthase family. GlcB subfamily.
XX
DR   Pfam; PF01274; Malate_synthase; 1; trigger=no
DR   NCBIfam; TIGR01345; Malate_syn_G; 1; trigger=no
XX
KW   Cytoplasm
KW   Transferase
KW   Glyoxylate bypass
KW   Magnesium
KW   Metal-binding
KW   Tricarboxylic acid cycle
KW   Oxidation
XX
GO   GO:0004474; F:malate synthase activity
GO   GO:0006097; P:glyoxylate cycle
GO   GO:0005737; C:cytoplasm
XX
FT   From: MASZ_ECOLI (P37330)
FT   BINDING         125..126
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT   BINDING         452..455
FT                   /ligand="glyoxylate"
FT                   /ligand_id="ChEBI:CHEBI:36655"
FT   ACT_SITE        338
FT                   /note="Proton acceptor"
FT   Condition: R
FT   ACT_SITE        631
FT                   /note="Proton donor"
FT   Condition: D
FT   BINDING         427
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: E
FT   BINDING         455
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Condition: D
FT   BINDING         118
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT   Condition: V
FT   BINDING         274
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT   Condition: S
FT   BINDING         311
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT   Condition: R
FT   BINDING         338
FT                   /ligand="glyoxylate"
FT                   /ligand_id="ChEBI:CHEBI:36655"
FT   Condition: R
FT   BINDING         427
FT                   /ligand="glyoxylate"
FT                   /ligand_id="ChEBI:CHEBI:36655"
FT   Condition: E
FT   BINDING         536
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT   Condition: P
FT   MOD_RES         617
FT                   /note="Cysteine sulfenic acid (-SOH)"
FT   Condition: C
FT   MOD_RES         688
FT                   /note="Cysteine sulfenic acid (-SOH)"
FT   Condition: C
XX
Size: 721-748;
Related: None;
Template: P42450; P37330; P9WK17;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: in PSESM
Plasmid: None
Comments: None
XX
# Revision 1.30 2023/06/01
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