AC   MF_00723;
DC   Protein; auto
c?   <OC:Bacteria>
TR   HAMAP; MF_00723; -; 1; level=0
XX
Names: Formald_GSH
XX
ID   GFA
DE   RecName: Full=Glutathione-dependent formaldehyde-activating enzyme;
DE            EC=4.4.1.22;
DE   AltName: Full=S-(hydroxymethyl)glutathione synthase;
GN   Name=gfa;
XX
CC   -!- FUNCTION: Catalyzes the condensation of formaldehyde and glutathione to
CC       S-hydroxymethylglutathione.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-(hydroxymethyl)glutathione = formaldehyde + glutathione;
CC         Xref=Rhea:RHEA:22488, ChEBI:CHEBI:16842, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:58758; EC=4.4.1.22;
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC       Note=Binds 2 Zn(2+) ions per subunit.;
end case
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (glutathione route): step 1/3.
CC   -!- SIMILARITY: Belongs to the Gfa family.
XX
DR   Pfam; PF04828; GFA; 1; trigger=no
DR   PIRSF; PIRSF033318; Formald_GSH; 1; trigger=no
DR   NCBIfam; TIGR02820; Formald_GSH; 1; trigger=no
DR   PROSITE; PS51891; CENP_V_GFA; 1; trigger=yes
XX
KW   Lyase
case <FTGroup:1>
KW   Metal-binding
KW   Zinc
end case
XX
GO   GO:0051907; F:S-(hydroxymethyl)glutathione synthase activity
GO   GO:0046294; P:formaldehyde catabolic process
case <FTGroup:1>
GO   GO:0008270; F:zinc ion binding
end case
XX
FT   From: GFA_PARDE (Q51669)
FT   BINDING         31
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /ligand_note="structural"
FT   Group: 1; Condition: C
FT   BINDING         33
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /ligand_note="structural"
FT   Group: 1; Condition: C
FT   BINDING         52
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /ligand_note="catalytic"
FT   Group: 1; Condition: C
FT   BINDING         54
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /ligand_note="catalytic"
FT   Group: 1; Condition: C
FT   BINDING         57
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /ligand_note="catalytic"
FT   Group: 1; Condition: C
FT   BINDING         99
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /ligand_note="structural"
FT   Group: 1; Condition: C
FT   BINDING         102
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /ligand_note="structural"
FT   Group: 1; Condition: C
XX
Size: 187-194;
Related: None;
Template: Q51669;
Scope:
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: in RHIME
Comments: None
XX
# Revision 1.31 2023/06/01
//