AC MF_00785; DC Protein; auto TR HAMAP; MF_00785; -; 1; level=0 XX Names: CbiX XX case or or or ID CFBA DE RecName: Full=Sirohydrochlorin cobaltochelatase; DE EC=4.99.1.3; DE AltName: Full=CbiXS; DE AltName: Full=Sirohydrochlorin nickelchelatase; DE EC=4.99.1.11; GN Name=cbiX; Synonyms=cfbA; else ID CBIX DE RecName: Full=Sirohydrochlorin cobaltochelatase; DE EC=4.99.1.3; DE AltName: Full=CbiXS; GN Name=cbiX; end case XX case or or or CC -!- FUNCTION: Catalyzes the insertion of Co(2+) into sirohydrochlorin as CC part of the anaerobic pathway to cobalamin biosynthesis. Involved in CC the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme CC F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which CC plays a key role in methanogenesis and anaerobic methane oxidation. CC Catalyzes the insertion of Ni(2+) into sirohydrochlorin to yield Ni- CC sirohydrochlorin. CC -!- CATALYTIC ACTIVITY: CC Reaction=Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin; CC Xref=Rhea:RHEA:15893, ChEBI:CHEBI:15378, ChEBI:CHEBI:48828, CC ChEBI:CHEBI:58351, ChEBI:CHEBI:60049; EC=4.99.1.3; CC -!- CATALYTIC ACTIVITY: CC Reaction=2 H(+) + Ni-sirohydrochlorin = Ni(2+) + sirohydrochlorin; CC Xref=Rhea:RHEA:52796, ChEBI:CHEBI:15378, ChEBI:CHEBI:49786, CC ChEBI:CHEBI:58351, ChEBI:CHEBI:136841; EC=4.99.1.11; else CC -!- FUNCTION: Catalyzes the insertion of Co(2+) into sirohydrochlorin as CC part of the anaerobic pathway to cobalamin biosynthesis. CC -!- CATALYTIC ACTIVITY: CC Reaction=Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin; CC Xref=Rhea:RHEA:15893, ChEBI:CHEBI:15378, ChEBI:CHEBI:48828, CC ChEBI:CHEBI:58351, ChEBI:CHEBI:60049; EC=4.99.1.3; end case CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis; CC cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): CC step 1/10. CC -!- SUBUNIT: Homotetramer; dimer of dimers. CC -!- SIMILARITY: Belongs to the CbiX family. CbiXS subfamily. XX DR Pfam; PF01903; CbiX; 1; trigger=no XX KW Cobalamin biosynthesis KW Lyase case or or or KW Methanogenesis end case case and or or or KW Metal-binding KW Cobalt KW Nickel else KW Metal-binding KW Cobalt end case XX GO GO:0016852; F:sirohydrochlorin cobaltochelatase activity GO GO:0019251; P:anaerobic cobalamin biosynthetic process case and or or or GO GO:0050897; F:cobalt ion binding GO GO:0016151; F:nickel cation binding else GO GO:0050897; F:cobalt ion binding end case XX FT From: CBIX_ARCFU (O29537) FT BINDING 69..74 FT /ligand="substrate" FT ACT_SITE 10 FT /note="Proton acceptor" FT Condition: H case or or or FT BINDING 10 FT /ligand="Co(2+)" FT /ligand_id="ChEBI:CHEBI:48828" FT Condition: H FT BINDING 10 FT /ligand="Ni(2+)" FT /ligand_id="ChEBI:CHEBI:49786" FT Condition: H FT BINDING 74 FT /ligand="Co(2+)" FT /ligand_id="ChEBI:CHEBI:48828" FT Condition: H FT BINDING 74 FT /ligand="Ni(2+)" FT /ligand_id="ChEBI:CHEBI:49786" FT Condition: H else FT BINDING 10 FT /ligand="Co(2+)" FT /ligand_id="ChEBI:CHEBI:48828" FT Condition: H FT BINDING 74 FT /ligand="Co(2+)" FT /ligand_id="ChEBI:CHEBI:48828" FT Condition: H end case FT BINDING 46 FT /ligand="substrate" FT Condition: [RKQE] XX Size: 120-144; Related: None; Template: O29537; O27448; P61816; Q8TJZ5; Scope: Archaea Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: HALSA has a protein with two tandem copies of a cbiX domain, resembling CbiK arrangement. XX # Revision 1.29 2022/11/19 //