AC MF_00802; DC Protein; auto TR HAMAP; MF_00802; -; 1; level=0 XX Names: GlnE XX ID GLNE DE RecName: Full=Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme; DE AltName: Full=ATP:glutamine synthetase adenylyltransferase; DE AltName: Full=ATase; DE Includes: DE RecName: Full=Glutamine synthetase adenylyl-L-tyrosine phosphorylase; DE EC=2.7.7.89; DE AltName: Full=Adenylyl removase; DE Short=AR; DE Short=AT-N; DE Includes: DE RecName: Full=Glutamine synthetase adenylyl transferase; DE EC=2.7.7.42; DE AltName: Full=Adenylyl transferase; DE Short=AT; DE Short=AT-C; GN Name=glnE; XX CC -!- FUNCTION: Involved in the regulation of glutamine synthetase GlnA, a CC key enzyme in the process to assimilate ammonia. When cellular nitrogen CC levels are high, the C-terminal adenylyl transferase (AT) inactivates CC GlnA by covalent transfer of an adenylyl group from ATP to specific CC tyrosine residue of GlnA, thus reducing its activity. Conversely, when CC nitrogen levels are low, the N-terminal adenylyl removase (AR) CC activates GlnA by removing the adenylyl group by phosphorolysis, CC increasing its activity. The regulatory region of GlnE binds the signal CC transduction protein PII (GlnB) which indicates the nitrogen status of CC the cell. CC -!- CATALYTIC ACTIVITY: CC Reaction=[glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + CC phosphate = [glutamine synthetase]-L-tyrosine + ADP; CC Xref=Rhea:RHEA:43716, Rhea:RHEA-COMP:10660, Rhea:RHEA-COMP:10661, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:46858, ChEBI:CHEBI:83624, CC ChEBI:CHEBI:456216; EC=2.7.7.89; CC -!- CATALYTIC ACTIVITY: CC Reaction=[glutamine synthetase]-L-tyrosine + ATP = [glutamine CC synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + diphosphate; CC Xref=Rhea:RHEA:18589, Rhea:RHEA-COMP:10660, Rhea:RHEA-COMP:10661, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:46858, CC ChEBI:CHEBI:83624; EC=2.7.7.42; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC -!- SIMILARITY: Belongs to the GlnE family. XX DR Pfam; PF03710; GlnE; 1; trigger=no XX KW ATP-binding KW Magnesium KW Multifunctional enzyme KW Nucleotide-binding KW Nucleotidyltransferase KW Transferase XX GO GO:0000287; F:magnesium ion binding GO GO:0005524; F:ATP binding GO GO:0008882; F:[glutamate-ammonia-ligase] adenylyltransferase activity GO GO:0000820; P:regulation of glutamine family amino acid metabolic process XX FT From: GLNE_ECOLI (P30870) FT REGION Nter..440 FT /note="Adenylyl removase" FT REGION 449..Cter FT /note="Adenylyl transferase" XX Size: 894-1076; Related: None; Template: P30870; Scope: Bacteria; Actinomycetota Bacteria; Pseudomonadota Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.20 2023/01/26 //