AC   MF_00817;
DC   Protein; auto
TR   HAMAP; MF_00817; -; 1; level=0
XX
Names: QueF_type2
XX
ID   QUEF
DE   RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase;
DE            EC=1.7.1.13;
DE   AltName: Full=7-cyano-7-carbaguanine reductase;
DE   AltName: Full=NADPH-dependent nitrile oxidoreductase;
DE   AltName: Full=PreQ(0) reductase;
GN   Name=queF;
XX
CC   -!- FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-
CC       deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-
CC         deazaguanine + 3 H(+) + 2 NADPH; Xref=Rhea:RHEA:13409,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:45075, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:58703; EC=1.7.1.13;
CC   -!- SUBUNIT: Homodimer.
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 2
CC       subfamily.
XX
DR   Pfam; PF01227; GTP_cyclohydroI; 1; trigger=no
DR   PIRSF; PIRSF004750; Nitrile_oxidored_YqcD_prd; 1; trigger=no
DR   NCBIfam; TIGR03138; QueF; 1; trigger=no
XX
KW   Cytoplasm
KW   NADP
KW   Oxidoreductase
KW   Queuosine biosynthesis
XX
GO   GO:0033739; F:preQ1 synthase activity
GO   GO:0008616; P:queuosine biosynthetic process
GO   GO:0006400; P:tRNA modification
GO   GO:0005737; C:cytoplasm
XX
FT   From: QUEF_VIBCH (Q9KTK0)
FT   BINDING         89..90
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Condition: S-[KQ]
FT   BINDING         256..257
FT                   /ligand="NADPH"
FT                   /ligand_id="ChEBI:CHEBI:57783"
FT   Condition: R-G
FT   BINDING         87..89
FT                   /ligand="substrate"
FT   Condition: [IV]-E-S
FT   BINDING         227..228
FT                   /ligand="substrate"
FT   Condition: [HA]-E
FT   ACT_SITE        188
FT                   /note="Thioimide intermediate"
FT   Condition: C
FT   ACT_SITE        195
FT                   /note="Proton donor"
FT   Condition: D
XX
Size: 262-289;
Related: MF_00818;
Template: Q46920; Q9KTK0;
Scope:
 Bacteria; Pseudomonadota
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.28 2024/01/31
//