AC   MF_00820;
DC   Protein; auto
TR   HAMAP; MF_00820; -; 1; level=0
XX
Names: Diketo_gul_reduc
XX
ID   DLGD
DE   RecName: Full=2,3-diketo-L-gulonate reductase;
DE            Short=2,3-DKG reductase;
DE            EC=1.1.1.130;
DE   AltName: Full=3-dehydro-L-gulonate 2-dehydrogenase;
GN   Name=dlgD;
XX
CC   -!- FUNCTION: Catalyzes the reduction of 2,3-diketo-L-gulonate in the
CC       presence of NADH, to form 3-keto-L-gulonate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-dehydro-L-gulonate + NAD(+) = 2,3-dioxo-L-gulonate + H(+) +
CC         NADH; Xref=Rhea:RHEA:21924, ChEBI:CHEBI:15378, ChEBI:CHEBI:57441,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57655, ChEBI:CHEBI:57945;
CC         EC=1.1.1.130;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-dehydro-L-gulonate + NADP(+) = 2,3-dioxo-L-gulonate + H(+) +
CC         NADPH; Xref=Rhea:RHEA:21928, ChEBI:CHEBI:15378, ChEBI:CHEBI:57441,
CC         ChEBI:CHEBI:57655, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.130;
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the LDH2/MDH2 oxidoreductase family. DlgD
CC       subfamily.
XX
DR   Pfam; PF02615; Ldh_2; 1; trigger=no
XX
KW   Cytoplasm
KW   NAD
KW   Oxidoreductase
XX
GO   GO:0047559; F:3-dehydro-L-gulonate 2-dehydrogenase activity
GO   GO:0005737; C:cytoplasm
XX
FT   From: DLGD_ECOLI (P37672)
FT   BINDING         168..174
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: x-x-M-x-D-x-S
FT   BINDING         224..225
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: W-K
FT   BINDING         304..306
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   Condition: G-x-E
FT   ACT_SITE        44
FT                   /note="Proton donor"
FT   Condition: H
XX
Size: 332-332;
Related: None;
Template: P37672;
Scope:
 Bacteria; Gammaproteobacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.16 2022/11/19
//