AC MF_00826; DC Protein; auto TR HAMAP; MF_00826; -; 1; level=0 XX Names: GRDA XX ID GRDA DE RecName: Full=Glycine/sarcosine/betaine reductase complex component A; DE EC=1.21.4.2; DE EC=1.21.4.3; DE EC=1.21.4.4; DE AltName: Full=Selenoprotein PA; DE AltName: Full=Thioredoxin reductase complex selenoprotein A; GN Name=grdA; XX CC -!- FUNCTION: In the first step of glycine, betaine and sarcosine CC reductases, the substrate is bound to component PB via a Schiff base CC intermediate. Then the PB-activated substrate is nucleophilically CC attacked by the selenol anion of component PA to transform it to a CC carboxymethylated selenoether and the respective amine. By action of CC component PC, acetyl phosphate is formed, leaving component PA in its CC oxidized state. Finally component PA becomes reduced by the thioredoxin CC system to start a new catalytic cycle of reductive deamination. CC -!- CATALYTIC ACTIVITY: CC Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + NH4(+) = CC [thioredoxin]-dithiol + glycine + H(+) + phosphate; CC Xref=Rhea:RHEA:12232, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:22191, CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29950, ChEBI:CHEBI:43474, CC ChEBI:CHEBI:50058, ChEBI:CHEBI:57305; EC=1.21.4.2; CC -!- CATALYTIC ACTIVITY: CC Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + methylamine CC = [thioredoxin]-dithiol + H(+) + phosphate + sarcosine; CC Xref=Rhea:RHEA:12825, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:22191, CC ChEBI:CHEBI:29950, ChEBI:CHEBI:43474, ChEBI:CHEBI:50058, CC ChEBI:CHEBI:57433, ChEBI:CHEBI:59338; EC=1.21.4.3; CC -!- CATALYTIC ACTIVITY: CC Reaction=[thioredoxin]-disulfide + acetyl phosphate + H2O + CC trimethylamine = [thioredoxin]-dithiol + glycine betaine + H(+) + CC phosphate; Xref=Rhea:RHEA:11848, Rhea:RHEA-COMP:10698, Rhea:RHEA- CC COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17750, CC ChEBI:CHEBI:22191, ChEBI:CHEBI:29950, ChEBI:CHEBI:43474, CC ChEBI:CHEBI:50058, ChEBI:CHEBI:58389; EC=1.21.4.4; CC -!- SUBUNIT: Monomer. Component of the glycine, sarcosine and betaine CC reductase complexes, together with components B and C. CC -!- SIMILARITY: Belongs to the GrdA family. XX DR Pfam; PF04723; GRDA; 1; trigger=no XX KW Oxidoreductase XX GO GO:0030699; F:glycine reductase activity GO GO:0050485; F:oxidoreductase activity, acting on X-H and Y-H to form an X-Y bond, with a disulfide as acceptor XX FT From: GRDA_ACESD (P26971) FT ACT_SITE 44 FT Condition: [CU] XX Size: 150-161; Related: None; Template: P26971; P52216; P50972; Scope: Bacteria Fusion: Nter: None Cter: None Duplicate: in PEPAC, PHOPR Plasmid: None Comments: None XX # Revision 1.17 2020/05/05 //