AC   MF_00916;
DC   Protein; auto
TR   HAMAP; MF_00916; -; 1; level=0
XX
Names: QueG
XX
ID   QUEG
DE   RecName: Full=Epoxyqueuosine reductase;
DE            EC=1.17.99.6;
DE   AltName: Full=Queuosine biosynthesis protein QueG;
GN   Name=queG;
XX
CC   -!- FUNCTION: Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine
CC       (Q), which is a hypermodified base found in the wobble positions of
CC       tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AH2 + epoxyqueuosine(34) in tRNA = A + H2O + queuosine(34) in
CC         tRNA; Xref=Rhea:RHEA:32159, Rhea:RHEA-COMP:18571, Rhea:RHEA-
CC         COMP:18582, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:17499,
CC         ChEBI:CHEBI:194431, ChEBI:CHEBI:194443; EC=1.17.99.6;
CC   -!- COFACTOR:
CC       Name=cob(II)alamin; Xref=ChEBI:CHEBI:16304;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 2 [4Fe-4S] clusters per monomer.
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:26230193}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the QueG family.
XX
DR   PROSITE; PS00198; 4FE4S_FER_1; 1; trigger=no
DR   PROSITE; PS51379; 4FE4S_FER_2; 1-2; trigger=yes
DR   Pfam; PF08331; DUF1730; 1; trigger=no
DR   Pfam; PF03130; HEAT_PBS; 0-1; trigger=yes
DR   NCBIfam; TIGR00276; TIGR00276; 1; trigger=no
XX
KW   4Fe-4S
KW   Cobalamin
KW   Cytoplasm
KW   Iron
KW   Iron-sulfur
KW   Metal-binding
KW   Oxidoreductase
KW   Queuosine biosynthesis
KW   tRNA processing
XX
GO   GO:0052693; F:epoxyqueuosine reductase activity
GO   GO:0051536; F:iron-sulfur cluster binding
GO   GO:0008616; P:queuosine biosynthetic process
GO   GO:0006400; P:tRNA modification
GO   GO:0005737; C:cytoplasm
XX
FT   From: QUEG_BACSU (P97030)
FT   BINDING         139..141
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Optional; Condition: S-[DE]-[RK]
FT   BINDING         240..241
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Condition: C-D
FT   ACT_SITE        134
FT                   /note="Proton donor"
FT   Condition: D
FT   BINDING         188
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT   Condition: C
FT   BINDING         191
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT   Condition: C
FT   BINDING         194
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT   Condition: C
FT   BINDING         198
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT   Condition: C
FT   BINDING         214
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT   Condition: C
FT   BINDING         240
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT   Condition: C
FT   BINDING         243
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT   Condition: C
FT   BINDING         247
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT   Condition: C
FT   BINDING         57
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Condition: R
FT   BINDING         97
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Optional; Condition: C
FT   BINDING         134
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Condition: D
FT   BINDING         152
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Optional; Condition: [ST]
FT   BINDING         155
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Optional; Condition: N
FT   BINDING         158
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Condition: [ILV]
FT   BINDING         169
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Condition: [LI]
FT   BINDING         216
FT                   /ligand="cob(II)alamin"
FT                   /ligand_id="ChEBI:CHEBI:16304"
FT   Optional; Condition: S
FT   BINDING         220
FT                   /ligand="tRNA"
FT                   /ligand_id="ChEBI:CHEBI:17843"
FT                   /ligand_part="tRNA anticodon stem loop"
FT   Optional; Condition: Q
FT   BINDING         222
FT                   /ligand="tRNA"
FT                   /ligand_id="ChEBI:CHEBI:17843"
FT                   /ligand_part="tRNA anticodon stem loop"
FT   Optional; Condition: K
FT   BINDING         280
FT                   /ligand="tRNA"
FT                   /ligand_id="ChEBI:CHEBI:17843"
FT                   /ligand_part="tRNA anticodon stem loop"
FT   Optional; Condition: N
FT   BINDING         281
FT                   /ligand="tRNA"
FT                   /ligand_id="ChEBI:CHEBI:17843"
FT                   /ligand_part="tRNA anticodon stem loop"
FT   Optional; Condition: [RK]
FT   BINDING         295
FT                   /ligand="tRNA"
FT                   /ligand_id="ChEBI:CHEBI:17843"
FT                   /ligand_part="tRNA anticodon stem loop"
FT   Optional; Condition: R
FT   BINDING         297
FT                   /ligand="tRNA"
FT                   /ligand_id="ChEBI:CHEBI:17843"
FT                   /ligand_part="tRNA anticodon stem loop"
FT   Optional; Condition: K
FT   BINDING         298
FT                   /ligand="tRNA"
FT                   /ligand_id="ChEBI:CHEBI:17843"
FT                   /ligand_part="tRNA anticodon stem loop"
FT   Optional; Condition: K
XX
Size: 289-411;
Related: None;
Template: P97030; P39288;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.14 2024/01/31
//