AC   MF_00983;
DC   Protein; auto
TR   HAMAP; MF_00983; -; 1; level=0
XX
Names: PriA
XX
ID   PRIA
case not <Feature:PS51192> and not <Feature:PS51194>
DE   RecName: Full=Probable replication restart protein PriA;
DE   AltName: Full=Putative ATP-dependent DNA helicase PriA;
else
DE   RecName: Full=Replication restart protein PriA;
DE   AltName: Full=ATP-dependent DNA helicase PriA;
DE            EC=5.6.2.4;
DE   AltName: Full=DNA 3'-5' helicase PriA;
end case
GN   Name=priA;
XX
CC   -!- FUNCTION: Initiates the restart of stalled replication forks, which
CC       reloads the replicative helicase on sites other than the origin of
CC       replication. Recognizes and binds to abandoned replication forks and
CC       remodels them to uncover a helicase loading site. Promotes assembly of
CC       the primosome at these replication forks.
case <Feature:PS51192> or <Feature:PS51194>
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC         translocating in the 3'-5' direction.; EC=5.6.2.4;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
end case
case <FTTag:zinc1> and <FTTag:zinc2>
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC       Note=Binds 2 zinc ions per subunit.;
end case
CC   -!- SUBUNIT: Component of the replication restart primosome.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
case not <Feature:PS51192> and not <Feature:PS51194>
CC   -!- CAUTION: As this protein does not have any detectable helicase domains,
CC       it probably does not have helicase activity.
end case
XX
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 0-1; trigger=yes
DR   PROSITE; PS51194; HELICASE_CTER; 0-1; trigger=yes
DR   Pfam; PF00270; DEAD; 1; trigger=no
DR   Pfam; PF00271; Helicase_C; 1; trigger=no
DR   NCBIfam; TIGR00595; priA; 1; trigger=no
XX
KW   ATP-binding
KW   DNA replication
KW   DNA-binding
case <Feature:PS51192> or <Feature:PS51194>
KW   Helicase
KW   Hydrolase
KW   Isomerase
end case
KW   Nucleotide-binding
KW   Primosome
case <FTTag:zinc1> and <FTTag:zinc2>
KW   Metal-binding
KW   Zinc
end case
XX
case <Feature:PS51192> or <Feature:PS51194>
GO   GO:0003678; F:DNA helicase activity
end case
GO   GO:0005524; F:ATP binding
GO   GO:0003677; F:DNA binding
case <FTTag:zinc1> and <FTTag:zinc2>
GO   GO:0008270; F:zinc ion binding
end case
GO   GO:0006260; P:DNA replication
GO   GO:1990077; C:primosome complex
XX
FT   From: PRIA_ECOLI (P17888)
FT   BINDING         436
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   Tag: zinc1; Condition: C
FT   BINDING         439
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   Tag: zinc1; Condition: C
FT   BINDING         445
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   Tag: zinc2; Condition: C
FT   BINDING         448
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   Tag: zinc2; Condition: C
FT   BINDING         463
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   Tag: zinc2; Condition: C
FT   BINDING         466
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   Tag: zinc2; Condition: C
FT   BINDING         476
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   Tag: zinc1; Condition: C
FT   BINDING         479
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   Tag: zinc1; Condition: C
XX
Size: 535-950;
Related: None;
Template: P17888; P94461; P0DXX8;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Version: 10
# Last updated date: 2025-02-04
# Created date: 2014-01-07
//