AC MF_01011; DC Protein; auto TR HAMAP; MF_01011; -; 1; level=0 XX Names: RNA_methyltr_TrmA XX ID TRMA DE RecName: Full=tRNA/tmRNA (uracil-C(5))-methyltransferase; DE EC=2.1.1.35; DE EC=2.1.1.-; DE AltName: Full=tRNA (uracil(54)-C(5))-methyltransferase; DE AltName: Full=tRNA(m5U54)-methyltransferase; DE Short=RUMT; DE AltName: Full=tmRNA (uracil(341)-C(5))-methyltransferase; GN Name=trmA; XX CC -!- FUNCTION: Dual-specificity methyltransferase that catalyzes the CC formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and CC that of position 341 (m5U341) in tmRNA (transfer-mRNA). CC -!- CATALYTIC ACTIVITY: CC Reaction=S-adenosyl-L-methionine + uridine(54) in tRNA = 5- CC methyluridine(54) in tRNA + H(+) + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:42712, Rhea:RHEA-COMP:10167, Rhea:RHEA-COMP:10193, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74447; EC=2.1.1.35; CC -!- CATALYTIC ACTIVITY: CC Reaction=S-adenosyl-L-methionine + uridine(341) in tmRNA = 5- CC methyluridine(341) in tmRNA + H(+) + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:43612, Rhea:RHEA-COMP:10630, Rhea:RHEA-COMP:10631, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74447; CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase CC superfamily. RNA M5U methyltransferase family. TrmA subfamily. XX DR Pfam; PF05958; tRNA_U5-meth_tr; 1; trigger=no DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1; trigger=no DR PROSITE; PS01230; TRMA_1; 1; trigger=no DR PROSITE; PS01231; TRMA_2; 1; trigger=no DR NCBIfam; TIGR02143; TrmA_only; 1; trigger=no XX KW Methyltransferase KW S-adenosyl-L-methionine KW Transferase KW tRNA processing XX GO GO:0030697; F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent GO GO:0030488; P:tRNA methylation XX FT From: TRMA_ECOLI (P23003) FT ACT_SITE 324 FT /note="Nucleophile" FT Condition: C FT ACT_SITE 358 FT /note="Proton acceptor" FT Condition: E FT BINDING 190 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: Q FT BINDING 218 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: Y FT BINDING 223 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: [NH] FT BINDING 239 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: E FT BINDING 299 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: D XX Size: 346-377; Related: MF_01010; Template: P23003; Scope: Bacteria; Pseudomonadota Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.36 2023/10/13 //