AC   MF_01037;
DC   Protein; auto
TR   HAMAP; MF_01037; -; 1; level=0
XX
Names: TrmFO
XX
ID   TRMFO
DE   RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO;
DE            EC=2.1.1.74;
DE   AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase;
DE   AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase;
GN   Name=trmFO;
XX
CC   -!- FUNCTION: Catalyzes the folate-dependent formation of 5-methyl-uridine
CC       at position 54 (M-5-U54) in all tRNAs.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH +
CC         uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-
CC         methyluridine(54) in tRNA + NAD(+); Xref=Rhea:RHEA:16873, Rhea:RHEA-
CC         COMP:10167, Rhea:RHEA-COMP:10193, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15636, ChEBI:CHEBI:57453, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74447; EC=2.1.1.74;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH +
CC         uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-
CC         methyluridine(54) in tRNA + NADP(+); Xref=Rhea:RHEA:62372, Rhea:RHEA-
CC         COMP:10167, Rhea:RHEA-COMP:10193, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15636, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74447; EC=2.1.1.74;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the MnmG family. TrmFO subfamily.
XX
DR   Pfam; PF01134; GIDA; 1; trigger=no
DR   NCBIfam; TIGR00137; Gid_trmFO; 1; trigger=no
DR   PROSITE; PS01280; GIDA_1; 1; trigger=no
DR   PROSITE; PS01281; GIDA_2; 1; trigger=no
XX
KW   Cytoplasm
KW   FAD
KW   Flavoprotein
KW   Methyltransferase
KW   NAD
KW   NADP
KW   Transferase
KW   tRNA processing
XX
GO   GO:0050660; F:flavin adenine dinucleotide binding
GO   GO:0047151; F:tRNA (uracil(54)-C5)-methyltransferase activity, 5,10-methylenetetrahydrofolate-dependent
GO   GO:0008033; P:tRNA processing
GO   GO:0005737; C:cytoplasm
XX
FT   From: TRMFO_BACSU (P39815)
FT   BINDING         10..15
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT   Condition: G-x-G-[LIM]-[AS]-G
XX
Size: 364-482;
Related: MF_00129;
Template: P39815; Q9S449;
Scope:
 Bacteria
Fusion:
 Nter: None
 Cter: None
Duplicate: in MESFL, MYCCT, MYCMS
Plasmid: None
Comments: None
XX
# Revision 1.34 2023/10/13
//