AC MF_01084; DC Protein; auto TR HAMAP; MF_01084; -; 1; level=0 XX Names: Diphthine_synth XX ID DPHB DE RecName: Full=Diphthine synthase; DE EC=2.1.1.98; DE AltName: Full=Diphthamide biosynthesis methyltransferase; GN Name=dphB; XX CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that CC catalyzes the trimethylation of the amino group of the modified target CC histidine residue in translation elongation factor 2 (EF-2), to form an CC intermediate called diphthine. The three successive methylation CC reactions represent the second step of diphthamide biosynthesis. CC -!- CATALYTIC ACTIVITY: CC Reaction=2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation CC elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine- CC [translation elongation factor 2] + 3 H(+) + 3 S-adenosyl-L- CC homocysteine; Xref=Rhea:RHEA:36415, Rhea:RHEA-COMP:9749, Rhea:RHEA- CC COMP:10172, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:73995, ChEBI:CHEBI:82696; EC=2.1.1.98; CC -!- PATHWAY: Protein modification; peptidyl-diphthamide biosynthesis. CC -!- SUBUNIT: Homodimer. CC -!- SIMILARITY: Belongs to the diphthine synthase family. XX DR Pfam; PF00590; TP_methylase; 1; trigger=no DR NCBIfam; TIGR00522; Dph5; 1; trigger=no DR PIRSF; PIRSF036432; Diphthine_synth; 1; trigger=no XX KW Transferase KW Methyltransferase KW S-adenosyl-L-methionine XX GO GO:0004164; F:diphthine synthase activity GO GO:0017183; P:protein histidyl modification to diphthamide XX FT From: DPHB_PYRHO (O58456) FT BINDING 115..116 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: [STN]-[IGVA] FT BINDING 10 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Optional; Condition: [LI] FT BINDING 87 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: D FT BINDING 90 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: [VALMTIG] FT BINDING 166 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: [LIV] FT BINDING 209 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Condition: [ALVM] FT BINDING 234 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT Optional; Condition: [HYQ] XX Size: 206-300; Related: None; Template: O58456; Q9YDI2; Scope: Archaea Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: Possible wrong C-terminal sequence in THEVO XX # Revision 1.25 2023/07/28 //